LOCUS       BBa_J119104             1849 bp    DNA     linear       22-Nov-2024
DEFINITION  pT7-RBS-GFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU
ACCESSION   BBa_J119104
VERSION     BBa_J119104.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     RBS             32..46
                     /label=RBS-1\Strong
     RBS             39..43
                     /label=RBS
     misc_feature    32..46
                     /label=BBa_B0030
     RBS             32..46
                     /label=BBa_B0030
     CDS             53..772
                     /label=GFP protein
     CDS             53..772
                     /label=BBa_E0040
     misc_feature    1..772
                     /label=BBa_K199176
     CDS             781..868
                     /label=end of LacI coding region (inactive)
     misc_feature    781..980
                     /label=BBa_R0010
     misc_feature    869..906
                     /label=CAP binding site
     promoter        917..922
                     /label=-35
     promoter        941..946
                     /label=-10
     misc_feature    946..980
                     /label=LacI binding site
     misc_feature    953..953
                     /label=start
     promoter        781..980
                     /label=BBa_R0010
     misc_feature    1034..1038
                     /label=5nt anti codon
     misc_feature    989..1131
                     /label=BBa_K199048
     CDS             1140..1227
                     /label=end of LacI coding region (inactive)
     misc_feature    1140..1339
                     /label=BBa_R0010
     misc_feature    1228..1265
                     /label=CAP binding site
     promoter        1276..1281
                     /label=-35
     promoter        1300..1305
                     /label=-10
     misc_feature    1305..1339
                     /label=LacI binding site
     misc_feature    1312..1312
                     /label=start
     promoter        1140..1339
                     /label=BBa_R0010
     misc_feature    1393..1397
                     /label=5 nt anticodon
     misc_feature    1348..1490
                     /label=BBa_K199014
     CDS             1499..1586
                     /label=end of LacI coding region (inactive)
     misc_feature    1499..1698
                     /label=BBa_R0010
     misc_feature    1587..1624
                     /label=CAP binding site
     promoter        1635..1640
                     /label=-35
     promoter        1659..1664
                     /label=-10
     misc_feature    1664..1698
                     /label=LacI binding site
     misc_feature    1671..1671
                     /label=start
     promoter        1499..1698
                     /label=BBa_R0010
     misc_feature    1752..1756
                     /label=5nt anti codon
     misc_feature    1707..1849
                     /label=BBa_K199046
     misc_feature    781..1849
                     /label=BBa_K199188
ORIGIN
        1 taatacgact cactataggg agatactaga gattaaagag gagaaatact agatgcgtaa
       61 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      121 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      181 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      241 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      301 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      361 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      421 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      481 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      541 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      601 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      661 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      721 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      841 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      901 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      961 gcggataaca atttcacaca tactagaggg atccaattcg gagagatgcc ggagcggctg
     1021 aacggaccgg tttggtggaa accggagtag gggcaactct accgggggtt caaatccccc
     1081 tctctccgcc actgcatatc cttagcgaaa gctaaggatt ttttttaagc ttactagagc
     1141 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1201 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1261 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1321 cggataacaa tttcacacat actagaggga tccaattcgg agagatgccg gagcggctga
     1381 acggaccggt ctgtcctaaa ccggagtagg ggcaactcta ccgggggttc aaatccccct
     1441 ctctccgcca ctgcatatcc ttagcgaaag ctaaggattt tttttaagct tactagagca
     1501 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1561 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     1621 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     1681 ggataacaat ttcacacata ctagagggat ccaattcgga gagatgccgg agcggctgaa
     1741 cggaccggtt tagtagatac cggagtaggg gcaactctac cgggggttca aatccccctc
     1801 tctccgccac tgcatatcct tagcgaaagc taaggatttt ttttaagct
//