LOCUS       BBa_J119118             2810 bp    DNA     linear       25-Nov-2024
DEFINITION  pLacI-RBS-T7RNAP with CUAGC frameshift mutation
ACCESSION   BBa_J119118
VERSION     BBa_J119118.1  
FEATURES             Location/Qualifiers
     misc_feature    116..120
                     /label=CTAGC frameshift
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggcgtc
       61 tcggcgaatt aaagaggaga aagtaggcat gaacacgaga gaccacgcga tcgcgctagc
      121 aggcgcgcca ggtctctgct ggaagacgtc acgattaaca tcgctaagaa cgacttctct
      181 gacatcgaac tggctgctat cccgttcaac actctagctg accattacgg tgagcgttta
      241 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
      301 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
      361 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
      421 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
      481 gccgtagcgt acatcaccat taagaccact ctagcttgcc taaccagtgc tgacaataca
      541 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
      601 cgtatccgtg accttgaagc taagcacttc aagaaaaacg tcgaggaaca actcaacaag
      661 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
      721 aagggtctac tcggtggcga ggcgtggtcc tcgtggcata aggaggactc tattcatgta
      781 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
      841 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
      901 gctatcgcaa cccgtgcagg ggcgctggct ggcatctctc cgatgttcca accttgcgta
      961 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
     1021 cctctagcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga ggacgtttac
     1081 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
     1141 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
     1201 cctgcgattg agcgtgaaga actcccgatg aaaccggagg acatcgacat gaatcctgag
     1261 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
     1321 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
     1381 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
     1441 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
     1501 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
     1561 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
     1621 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
     1681 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
     1741 ccgctggcgt ttgacgggtc ttgctccggc atccagcact tctccgcgat gctccgagat
     1801 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
     1861 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
     1921 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
     1981 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
     2041 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
     2101 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
     2161 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
     2221 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
     2281 aagaaaactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
     2341 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
     2401 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
     2461 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
     2521 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
     2581 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
     2641 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
     2701 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
     2761 cgtgacatct tagagtcgga cttcgcgttc gcgtaataac gcatgagacg
//