LOCUS       BBa_J119122              866 bp    DNA     linear       23-Dec-2024
DEFINITION  J119044+J23100 Vector
ACCESSION   BBa_J119122
VERSION     BBa_J119122.1  
FEATURES             Location/Qualifiers
     promoter        34..68
                     /label=J23100 Promoter
     misc_feature    69..167
                     /label=BD18 bicistron
     RBS             97..105
                     /label=Leader RBS
     RBS             150..158
                     /label=GOI RBS
     misc_feature    163..165
                     /label=Stop for BD18
     CDS             165..837
                     /label=RFP E1010 with 4 pt mutations
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag accttgacgg ctagctcagt cctaggtaca
       61 gtgctagcgg tctcagcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      121 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggct tcctccgaag
      181 atgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      241 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      301 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      361 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
      421 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagatggt ggtgttgtta
      481 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
      541 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
      601 ccaccgaacg tatgtacccg gaagatggtg ctctgaaagg tgaaatcaaa atgcgtctga
      661 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
      721 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
      781 aagattacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      841 aataatacta gtagcggccg ctgcag
//