##gff-version 3
##sequence-region BBa_J119122 34 837
BBa_J119122	.	CDS	165	837	.	+	0	ID=annotation2177587;Name=RFP E1010 with 4 pt mutations
BBa_J119122	.	ribosome_entry_site	97	105	.	+	0	ID=annotation2177584;Name=Leader RBS
BBa_J119122	.	sequence_feature	69	167	.	+	0	ID=annotation2177583;Name=BD18 bicistron
BBa_J119122	.	stop_codon	163	165	.	+	0	ID=annotation2177586;Name=Stop for BD18
BBa_J119122	.	promoter	34	68	.	+	0	ID=annotation2177582;Name=J23100 Promoter
BBa_J119122	.	ribosome_entry_site	150	158	.	+	0	ID=annotation2177585;Name=GOI RBS
>BBa_J119122
gaattcgcggccgcttctagagcgactgagaccttgacggctagctcagtcctaggtacagtgctagcggtctcagcgg
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcg
tttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtc
acgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaagg
tggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggct
gacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtg
ttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttccc
gtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgct
ctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatgg
ctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacac
catcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcag