LOCUS       BBa_J119127              883 bp    DNA     linear       28-Sep-2024
DEFINITION  For Testing New Promoters via Golden Gate Assembly with BsmBI
ACCESSION   BBa_J119127
VERSION     BBa_J119127.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=BsmBI sticky end left
     misc_feature    6..11
                     /label=BsmBI cuts left
     stem_loop       12..106
                     /label=TT B0014
     misc_feature    107..112
                     /label=BsmBI cuts right
     misc_feature    114..117
                     /label=BsmBI sticky end right
     misc_feature    118..205
                     /label=BD18 biscistron
     RBS             135..143
                     /label=leader RBS
     RBS             188..196
                     /label=RBS for RFP
     misc_feature    201..203
                     /label=Stop for BD18 leader
     CDS             203..883
                     /label=RFP E1010
     misc_feature    203..205
                     /label=Start for RFP
     misc_feature    878..883
                     /label=Double stop for E1010
ORIGIN
        1 cgactgagac gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
       61 agagaatata aaaagccaga ttattaatcc ggctttttta ttatttcgtc tcagcggggg
      121 cccaagttca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta
      181 atcatgcgac ggagcgtttc taatggcttc ctccgaagat gttatcaaag agttcatgcg
      241 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      301 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      361 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      421 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      481 ggaacgtgtt atgaacttcg aagatggtgg tgttgttacc gttacccagg actcctccct
      541 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      601 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      661 agatggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      721 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      781 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gattacacca tcgttgaaca
      841 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taa
//