BBa_J119129
1
BBa_J119129
pTac with mutations
2013-01-07T12:00:00Z
2015-08-31T04:08:27Z
Oligonucleotides.
This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. A mutation was created by removing the ATTA sequence after the -35 location. This had the effect of reducing the spacing between the -35 and -10 elements from 16 base pairs to 12 base pairs.
false
false
_613_435_
0
606
61
Not in stock
false
The oligonucleotides needed to have sticky ends compatible with those produced by BsaI digestion of J100091 so that Golden Gate Assembly could be used.
false
MWSU Genetics Lab Fall 2012 Tues 8:00
annotation2213801
1
-35 region
range2213801
1
11
16
annotation2213802
1
-10 region
range2213802
1
29
34
BBa_J119129_sequence
1
cgacgagctgttgacaatcatcggctcgtataatgtgtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z