LOCUS       BBa_J119136             1632 bp    DNA     linear       22-Nov-2024
DEFINITION  Device for Testing New Promoters via Golden Gate Assembly
ACCESSION   BBa_J119136
VERSION     BBa_J119136.1  
FEATURES             Location/Qualifiers
     RBS             727..741
                     /label=RBS reverse
     CDS             1..719
                     /label=GFP reverse
     misc_feature    1..741
                     /label=BBa_J119135
     misc_feature    750..753
                     /label=BsmBI sticky end left
     misc_feature    755..760
                     /label=BsmBI cuts left
     stem_loop       761..855
                     /label=TT B0014
     misc_feature    856..861
                     /label=BsmBI cuts right
     misc_feature    863..866
                     /label=BsmBI sticky end right
     misc_feature    867..954
                     /label=BD18 biscistron
     RBS             884..892
                     /label=leader RBS
     RBS             937..945
                     /label=RBS for RFP
     misc_feature    950..952
                     /label=Stop for BD18 leader
     CDS             952..1632
                     /label=RFP E1010
     misc_feature    952..954
                     /label=Start for RFP
     misc_feature    1627..1632
                     /label=Double stop for E1010
     misc_feature    750..1632
                     /label=BBa_J119127
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa ttactagagc gactgagacg tcacactggc tcaccttcgg
      781 gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg
      841 gcttttttat tatttcgtct cagcgggggc ccaagttcac ttaaaaagga gatcaacaat
      901 gaaagcaatt ttcgtactga aacatcttaa tcatgcgacg gagcgtttct aatggcttcc
      961 tccgaagatg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
     1021 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1081 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1141 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1201 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agatggtggt
     1261 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1321 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1381 gaagcttcca ccgaacgtat gtacccggaa gatggtgctc tgaaaggtga aatcaaaatg
     1441 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1501 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1561 cacaacgaag attacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1621 ggtgcttaat aa
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