LOCUS       BBa_J119137             1625 bp    DNA     linear       26-Nov-2024
DEFINITION  pClone Red - Device for Testing New Promoters via Golden Gate 
            Assembly
ACCESSION   BBa_J119137
VERSION     BBa_J119137.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse
     RBS             727..738
                     /label=B0030 RBS reverse
     misc_feature    755..758
                     /label=Sticky end 
     misc_feature    760..765
                     /label=BsaI left
     promoter        766..836
                     /label=pLacIQ1 reverse
     misc_feature    837..842
                     /label=BsaI right
     misc_feature    844..847
                     /label=Sticky end
     RBS             860..944
                     /label=BD18 bicistron RBS
     CDS             945..1625
                     /label=RFP
     misc_feature    1620..1625
                     /label=double stop
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactg agaccccggg cgctatcatg
      781 ccataccgcg aaaggttttg caccattcga tggtgtcaac gtaaatgcat gccgctggtc
      841 tctgcgggaa gacaactagg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      901 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggct tcctccgaag
      961 atgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     1021 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
     1081 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     1141 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     1201 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagatggt ggtgttgtta
     1261 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     1321 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1381 ccaccgaacg tatgtacccg gaagatggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1441 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     1501 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     1561 aagattacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     1621 aataa
//