LOCUS       BBa_J119138             1634 bp    DNA     linear       28-Sep-2024
DEFINITION  Device for Testing New Promoters via Golden Gate Assembly
ACCESSION   BBa_J119138
VERSION     BBa_J119138.1  
FEATURES             Location/Qualifiers
     misc_feature    23..26
                     /label=Sticky end
     misc_feature    843..846
                     /label=Sticky end
     misc_feature    28..33
                     /label=BsaI left
     misc_feature    849..855
                     /label=BsaI right
     CDS             34..753
                     /label=GFP reverse
     RBS             760..772
                     /label=RBS reverse
     promoter        781..836
                     /label=pLacIQ1 reverse
     CDS             933..1613
                     /label=RFP
     RBS             848..935
                     /label=BD18 bicistron RBS
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag accttattat ttgtatagtt catccatgcc
       61 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
      121 gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta aaaggacagg
      181 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc ttccatcttc
      241 aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt ctgccatgat
      301 gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc
      361 tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt caaacttgac
      421 ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata
      481 accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg ggtatctcgc
      541 aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa caggtagttt
      601 tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc
      661 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      721 aactccagtg aaaagttctt ctcctttacg catctagtac tttcctgtgt gactctagta
      781 ccgggcgcta tcatgccata ccgcgaaagg tggtgtcaac gtaaatgcat gccgctggtc
      841 tctgcggggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg
      901 aaacatctta atcatgcgac ggagcgtttc taatggcttc ctccgaagat gttatcaaag
      961 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     1021 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     1081 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     1141 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     1201 gtttcaaatg ggaacgtgtt atgaacttcg aagatggtgg tgttgttacc gttacccagg
     1261 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     1321 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     1381 tgtacccgga agatggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     1441 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     1501 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gattacacca
     1561 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taatactagt
     1621 agcggccgct gcag
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