LOCUS       BBa_J119139             2359 bp    DNA     linear       10-Dec-2025
DEFINITION  Device for Testing New Promoters via Golden Gate Assembly
ACCESSION   BBa_J119139
VERSION     BBa_J119139.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse
     RBS             727..741
                     /label=RBS reverse
     misc_feature    772..775
                     /label=Sticky end
     misc_feature    1590..1593
                     /label=Sticky end
     RBS             1594..1678
                     /label=BD18 bicistron RBS
     CDS             1679..2359
                     /label=RFP
     RBS             1452..1536
                     /label=BD18 bicistron RBS
     CDS             783..1451
                     /label=ampil CP (blue)
     promoter        1536..1582
                     /label=P2 (strong)
     misc_feature    1583..1588
                     /label=BsaI right
     misc_feature    777..782
                     /label=BsaI left
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa ttactagagg aattcgcggc cgcttctaga gcgactgaga
      781 ccttattagg caacaaccgg tttacgtgca atgctaattt cgcactgttc aacgctggtg
      841 taatctttat tgtggttggt cacatccagt ttacgatcca cataatgata accaggcatt
      901 ttcaccggtt ttttggcttt gtaggtggtt ttaaactcac acagataatg accaccacct
      961 tccagtttca gtgccataaa gttattaccc agcagcatgc catcacgtgc aaacagacgt
     1021 tcggtattcg gttcccaacc ctgggttttt ttctgcataa ccggaccatt aggcggaaaa
     1081 ttcagaccgc taaatttcac gtggtagata aagcaattac cctgaatgct gctatcattg
     1141 ctaacggtgc aaactgcacc atcttcaaag ttcataatac gttcccaggt ataaccttcc
     1201 ggaaagctct gtttcacata atccgggata tcttccggat atttggtaaa cggaatgcta
     1261 ccatactgac actgcggact cagaatatcc catgcaaacg gcagcggacc acctttggta
     1321 acggtcagtt taacggtctg ttcgccttca tacggtttgc ctttaccatc accttcaact
     1381 tcaaaataat ggccgttcac ggtgccgctc atatacactt tataggtcat ctgctttgca
     1441 atcacgctca ttagaaacgc tccgtcgcat gattaagatg tttcagtacg aaaattgctt
     1501 tcattgttga tctccttttt aagtgaactt gggccctcca cacattatag gtacaaaaag
     1561 atgcgaagtc aatactcttt ttggtctctg cgggggccca agttcactta aaaaggagat
     1621 caacaatgaa agcaattttc gtactgaaac atcttaatca tgcgacggag cgtttctaat
     1681 ggcttcctcc gaagatgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
     1741 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
     1801 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
     1861 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
     1921 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
     1981 tggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
     2041 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
     2101 gggttgggaa gcttccaccg aacgtatgta cccggaagat ggtgctctga aaggtgaaat
     2161 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
     2221 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
     2281 cacctcccac aacgaagatt acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
     2341 ctccaccggt gcttaataa
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