##gff-version 3
##sequence-region BBa_J119139 1 2359
BBa_J119139	.	ribosome_entry_site	727	741	.	+	0	ID=annotation2214111;Name=RBS reverse
BBa_J119139	.	ribosome_entry_site	1452	1536	.	+	0	ID=annotation2214116;Name=BD18 bicistron RBS
BBa_J119139	.	CDS	1	720	.	+	0	ID=annotation2214110;Name=GFP reverse
BBa_J119139	.	ribosome_entry_site	1594	1678	.	+	0	ID=annotation2214114;Name=BD18 bicistron RBS
BBa_J119139	.	promoter	1536	1582	.	+	0	ID=annotation2214118;Name=P2 (strong)
BBa_J119139	.	sequence_feature	777	782	.	+	0	ID=annotation2214120;Name=BsaI left
BBa_J119139	.	sequence_feature	1583	1588	.	+	0	ID=annotation2214119;Name=BsaI right
BBa_J119139	.	CDS	1679	2359	.	+	0	ID=annotation2214115;Name=RFP
BBa_J119139	.	sequence_feature	1590	1593	.	+	0	ID=annotation2214113;Name=Sticky end
BBa_J119139	.	CDS	783	1451	.	+	0	ID=annotation2214117;Name=ampil CP (blue)
BBa_J119139	.	sequence_feature	772	775	.	+	0	ID=annotation2214112;Name=Sticky end
>BBa_J119139
ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctc
ttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaa
ttggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaacttt
gattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatg
tttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcac
gtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaa
gtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccat
ggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccac
tgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcc
tttacgcatctagtatttctcctctttaattactagaggaattcgcggccgcttctagagcgactgagaccttattagg
caacaaccggtttacgtgcaatgctaatttcgcactgttcaacgctggtgtaatctttattgtggttggtcacatccag
tttacgatccacataatgataaccaggcattttcaccggttttttggctttgtaggtggttttaaactcacacagataa
tgaccaccaccttccagtttcagtgccataaagttattacccagcagcatgccatcacgtgcaaacagacgttcggtat
tcggttcccaaccctgggtttttttctgcataaccggaccattaggcggaaaattcagaccgctaaatttcacgtggta
gataaagcaattaccctgaatgctgctatcattgctaacggtgcaaactgcaccatcttcaaagttcataatacgttcc
caggtataaccttccggaaagctctgtttcacataatccgggatatcttccggatatttggtaaacggaatgctaccat
actgacactgcggactcagaatatcccatgcaaacggcagcggaccacctttggtaacggtcagtttaacggtctgttc
gccttcatacggtttgcctttaccatcaccttcaacttcaaaataatggccgttcacggtgccgctcatatacacttta
taggtcatctgctttgcaatcacgctcattagaaacgctccgtcgcatgattaagatgtttcagtacgaaaattgcttt
cattgttgatctcctttttaagtgaacttgggccctccacacattataggtacaaaaagatgcgaagtcaatactcttt
ttggtctctgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatc
atgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggt
tccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactga
aagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgt
taaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttc
gaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtg
gtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtaccc
ggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaca
acgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa