LOCUS       BBa_J119141             1652 bp    DNA     linear       22-Dec-2024
DEFINITION  Device for Testing New Promoters via BsgI Golden Gate Assembly
ACCESSION   BBa_J119141
VERSION     BBa_J119141.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP
     RBS             726..741
                     /label=RBS
     misc_feature    743..745
                     /label=BsaI Sticky Ends
     misc_feature    757..763
                     /label=BsgI
     promoter        765..800
                     /label=J23100
     misc_feature    801..806
                     /label=BsgI
     misc_feature    821..822
                     /label=BsaI Sticky Ends
     RBS             823..906
                     /label=BD18 bicistroin RBS
     CDS             907..1614
                     /label=RFP
     misc_feature    1609..1615
                     /label=Double Stop
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa ttacgactgg atagactgac tgcacttgac ggctagctca
      781 gtcctaggta cagtgctagc gtgcagccag actttcacgc gggggcccaa gttcacttaa
      841 aaaggagatc aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gcgacggagc
      901 gtttctaatg gcttcctccg aagatgttat caaagagttc atgcgtttca aagttcgtat
      961 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
     1021 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1081 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1141 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1201 cttcgaagat ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1261 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1321 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagatg gtgctctgaa
     1381 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1441 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1501 actggacatc acctcccaca acgaagatta caccatcgtt gaacagtacg aacgtgctga
     1561 aggtcgtcac tccaccggtg cttaataata ctagtagcgg ccgctgcagt actagagttg
     1621 acggctagct cagtcctagg tacagtgcta gc
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