BBa_J119141 1 BBa_J119141 Device for Testing New Promoters via BsgI Golden Gate Assembly 2013-04-14T11:00:00Z 2015-08-31T04:08:27Z Groningen 2009, Warsaw 2010, Claire Shinneman, Paul Frederick Group: iGEM2006_Davidson This part will allow users to clone and test new promoters without gel purification or other preparation of DNA - it is ideal for teaching labs. It is a destination vector for Golden Gate Assembly (GGA) using BsgI and Ligase. A new promoter can be derived from synthetic oligos, PCR, or a plasmid clones. The new promoter must be flanked by BsgI sites that produce the 2 nt overhangs required for assembly. The left site must be 5' CTGCAC 3' and the right site must be 5' GTGCAG 3'. Successful GGA assembly replaces the reverve promoter driving RFP expression with the new promoter. The new promoter will drive RFP expression in the forward direction or GFP expression if it has reverse promoter function. The part incorporation of J23100 promoter was designed by Warsaw 2010. false false _613_435_ 0 606 61 Not in stock false J119137 (Plasmid) + J23100 (Promoter) false Todd Eckdahl, Claire Shinneman annotation2215125 1 GFP range2215125 1 1 720 annotation2215126 1 RBS range2215126 1 726 741 annotation2215129 1 J23100 range2215129 1 765 800 annotation2215131 1 BsaI Sticky Ends range2215131 1 821 822 annotation2215134 1 Double Stop range2215134 1 1609 1615 annotation2215127 1 BsaI Sticky Ends range2215127 1 743 745 annotation2215130 1 BsgI range2215130 1 801 806 annotation2215132 1 BD18 bicistroin RBS range2215132 1 823 906 annotation2215128 1 BsgI range2215128 1 757 763 annotation2215133 1 RFP range2215133 1 907 1614 BBa_J119141_sequence 1 ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaattacgactggatagactgactgcacttgacggctagctcagtcctaggtacagtgctagcgtgcagccagactttcacgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcagtactagagttgacggctagctcagtcctaggtacagtgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z