LOCUS       BBa_J119142              786 bp    DNA     linear       15-Jun-2024
DEFINITION  Part for inserting modified D-Dogs for Golden Gate Assembly using 
            BsgI
ACCESSION   BBa_J119142
VERSION     BBa_J119142.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=Sticky word
     misc_feature    6..11
                     /label=BsaI (left)
     promoter        12..45
                     /label=J119030
     misc_feature    48..53
                     /label=BsaI (right)
     misc_feature    55..58
                     /label=Sticky word
     misc_feature    59..60
                     /label=BsgI sticky word (left)
     misc_feature    59..106
                     /label="Stuffer" (non-functional)
     misc_feature    75..80
                     /label=BsgI (left)
     misc_feature    85..90
                     /label=BsgI (right)
     misc_feature    105..106
                     /label=BsgI Sticky word (right)
     CDS             106..786
                     /label=mRFP
     misc_feature    106..108
                     /label=Start Codon
     misc_feature    781..783
                     /label=Stop
     misc_feature    784..786
                     /label=Stop
ORIGIN
        1 cgaccgagac cttgacatca ggaaaatttt tctgtataat gtgtggaggt ctcagcggca
       61 actggctcac cttcctgcac gcatgtgcag gggtgggcct ttcgaatggc ttcctccgaa
      121 gatgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac
      181 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa
      241 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc
      301 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg
      361 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagatgg tggtgttgtt
      421 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt
      481 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct
      541 tccaccgaac gtatgtaccc ggaagatggt gctctgaaag gtgaaatcaa aatgcgtctg
      601 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa
      661 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
      721 gaagattaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
      781 taataa
//