LOCUS       BBa_J100091              926 bp    DNA     linear       01-Jun-2024
DEFINITION  For Testing New Promoters via Golden Gate Assembly
ACCESSION   BBa_J100091
VERSION     BBa_J100091.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa Prefix
     misc_feature    23..26
                     /label=BsaI sticky end left
     misc_feature    28..33
                     /label=BsaI cuts left
     stem_loop       34..128
                     /label=TT B0014
     misc_feature    129..134
                     /label=BsaI cuts right
     misc_feature    136..139
                     /label=BsaI sticky end right
     misc_feature    140..227
                     /label=BD18 bicistron
     RBS             157..165
                     /label=leader RBS
     RBS             210..218
                     /label=RBS for RFP
     misc_feature    223..225
                     /label=Stop for BD18 leader
     misc_feature    225..227
                     /label=Start for RFP
     CDS             225..905
                     /label=RFP E1010
     misc_feature    900..905
                     /label= Double Stop for E1010
     misc_feature    906..926
                     /label=BBa suffix
     primer_bind     1..20
                     /label=PCR For Primer
     primer_bind     232..251
                     /label=PCR Rev Primer
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag acctcacact ggctcacctt cgggtgggcc
       61 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
      121 tattatttgg tctcagcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      181 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggct tcctccgaag
      241 atgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      301 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      361 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      421 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
      481 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagatggt ggtgttgtta
      541 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
      601 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
      661 ccaccgaacg tatgtacccg gaagatggtg ctctgaaagg tgaaatcaaa atgcgtctga
      721 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
      781 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
      841 aagattacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      901 aataatacta gtagcggccg ctgcag
//
LOCUS       BBa_J119300              926 bp    DNA     linear       01-Jun-2024
DEFINITION  Part for inserting modified D-Dogs for Golden Gate Assembly using 
            BsgI
ACCESSION   BBa_J119300
VERSION     BBa_J119300.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa Prefix
     misc_feature    23..26
                     /label=BsaI sticky end left
     misc_feature    28..33
                     /label=BsaI cuts left
     stem_loop       34..128
                     /label=TT B0014
     misc_feature    129..134
                     /label=BsaI cuts right
     misc_feature    136..139
                     /label=BsaI sticky end right
     misc_feature    140..227
                     /label=BD18 bicistron
     RBS             157..165
                     /label=leader RBS
     RBS             210..218
                     /label=RBS for RFP
     misc_feature    223..225
                     /label=Stop for BD18 leader
     misc_feature    225..227
                     /label=Start for RFP
     CDS             225..905
                     /label=RFP E1010
     misc_feature    900..905
                     /label= Double Stop for E1010
     misc_feature    906..926
                     /label=BBa suffix
     misc_feature    1..926
                     /label=BBa_J100091
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag acctcacact ggctcacctt cgggtgggcc
       61 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
      121 tattatttgg tctcagcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      181 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggct tcctccgaag
      241 atgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      301 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      361 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      421 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
      481 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagatggt ggtgttgtta
      541 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
      601 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
      661 ccaccgaacg tatgtacccg gaagatggtg ctctgaaagg tgaaatcaaa atgcgtctga
      721 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
      781 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
      841 aagattacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      901 aataatacta gtagcggccg ctgcag
//