LOCUS       BBa_J119304             3277 bp    DNA     linear       22-Dec-2024
DEFINITION  eCDM8 expression with low promoter and low C dog RBS
ACCESSION   BBa_J119304
VERSION     BBa_J119304.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=J119030
     RBS             37..124
                     /label=J119025
     misc_feature    122..3277
                     /label=eCDM8
ORIGIN
        1 ttgacatcag gaaaattttt ctgtataatg tgtggagggc ccaagttcac ttaaaaagga
       61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gaggttttct
      121 aatgatgacc atcaaagaaa tgccgcagcc gaaaaccttt ggtgaactga aaaatctgcc
      181 gctgctgaat accgataaac cggttcatgc actgatgaaa attgcagatg aactgggcga
      241 gatctttaaa ttcgaagcac cgggtagcgt tacccgttat ctgagcagcc agcgtctgat
      301 taaagaagca tgcgacgaaa gccgctttga taaaaatctg attcagtggc tgaaattcat
      361 ccgcgatttt ctgggtgatg gtctgagcac cagctggacc catgaaaaaa actggaaaaa
      421 agcccataat attctgctgc cgagctttag ccagcaggca atgaaaggtt atcatgcaat
      481 gatggttgat attgccgttc agctggttca gaaatgggaa cgtctgaatg cagatgaaca
      541 tattgaagtt ccggaagata tgacccgtct gaccctggat accattggtc tgtgtggttt
      601 taactatcgc ttcaacagct tttatcgtga tcagccgcat ccgtttgtta ccagcatggt
      661 tcgtgcactg gatgaagcag tgaataaatg gcagcgtgca aatccggatg atccggcata
      721 tgatgaaaat aaacgtcagt tccaagagga tattaaagtg atgaatgacc tggtggacaa
      781 aattatcgca gatcgtaaag caagcggtga acagagtgat gatctgctga cccatatgct
      841 gaatggtaaa gatccggaaa ccggtgaacc gctggatgat gaaaacattc gttatcagat
      901 tattaccttc ctgattgccg gtcatgaaac caccagtggt ctgctgagct ttgcactgta
      961 ttttctggtt aaaaatccgc acgttctgca aaaagcagcc gaagaagcag cacgcgttct
     1021 ggttgatccg gttccgagct ataaacaggt taaacagctg aaatatgtgg gcatggttct
     1081 gaatgaagca ctgcgtctgt ggccgacctt tccgtggttt agcctgtatg caaaagaaga
     1141 taccgttctg ggaggtgaat atccgctgga aaaaggtgat gaactgatgg ttctgattcc
     1201 gcagctgcat cgtgataaaa ccatttgggg tgatgatgtg gaagaatttc gtccggaacg
     1261 ttttgaaaat ccgagcgcaa ttccgcagca tgcctttaaa ccgtttggta atggtcagcg
     1321 tgcatgtatt ggtcagcagt ttgcactgca tgaagccacc ctggttctgg gtatgatgct
     1381 gaaacatttt gattttgagg accacaccaa ctatgagctg gatattaaag gcaccctgac
     1441 cctgaaaccg gaaggttttg ttgttaaagc caaaagcaaa aaaatcccgc tgggtggtat
     1501 tccgagcccg agcaccgaac agagcgcaaa aaaagttcgt aaaaaagccg aaaacgccca
     1561 taatacaccg ctgctggttc tgtatggtag caatatgggt acagccgaag gcaccgcacg
     1621 tgatctggca gatattgcaa tgagcaaagg ttttgcaccg caggttgcaa ccctggatag
     1681 ccatgcaggt aatctgcctc gtgaaggtgc agttctgatt gttaccgcaa gctataatgg
     1741 tcatccgcct gataatgcaa aacagtttgt tgattggctg gatcaggcaa gcgcagatga
     1801 agttaaaggt gttcgttata gcgtttttgg ttgcggtgat aaaaactggg caaccaccta
     1861 tcagaaagtt ccggcattta ttgatgaaac cctggcagca aaaggtgcag aaaatattgc
     1921 agatcgtggt gaaaccgatg ccagtgatga ttttgaaggc acctatgaag aatggcgtga
     1981 acatatgtgg tcagatgttg cagcctattt taacctggat atcgaaaaca gcgaggacaa
     2041 taaaagcacc ctgagcctgc aatttgtgga tagcgcagca gatatgccgc tggcaaaaat
     2101 gcatggtgca tttagcacca atgttgttgc aagcaaagaa ctgcaacagc ctggtagcgc
     2161 acgtagcacc cgtcatctgg aaattgaact gccgaaagaa gcaagctatc aagagggtga
     2221 tcatctgggt gttattccgc gtaattatga aggtattgtt aatcgtgtta ccgcacgttt
     2281 tggtctggat gcaagccagc agattcgtct ggaagcagaa gaagaaaaac tggcacatct
     2341 gccgctggcc aaaaccgtta gcgttgaaga actgctgcaa tatgttgaac tgcaagatcc
     2401 ggttacccgt acccagctgc gtgcaatggc agcaaaaacc gtttgtccgc ctcataaagt
     2461 tgaactggaa gcactgctgg agaaacaggc atataaagaa caggttctgg caaaacgtct
     2521 gaccatgctg gaactgctgg aaaaatatcc ggcatgcgaa atgaaattca gcgaatttat
     2581 tgcactgctg ccgagtattc gtccgcgtta ttatagcatt agcagcagtc cgcgtgttga
     2641 tgaaaaacag gccagcatta ccgttagcgt ggttagcggt gaagcatggt caggttatgg
     2701 tgaatataaa ggtattgcca gcaattatct ggccgaactg caagaaggtg ataccattac
     2761 ctgttttatt agcacaccgc agagcgaatt taccctgccg aaagatcctg aaacaccgct
     2821 gattatggtt ggtccgggta caggtgttgc accgtttcgt ggttttgttc aggcacgtaa
     2881 acaactgaaa gaacagggtc agagcctggg tgaagcacat ctgtattttg gttgtcgtag
     2941 tccgcatgag gattatctgt atcaagaaga actggaaaac gcacagagcg aaggtattat
     3001 taccctgcat accgcattta gccgtatgcc gaatcagccg aaaacatatg ttcagcatgt
     3061 tatggaacag gatggcaaaa aactgattga actgctggat cagggtgccc atttctatat
     3121 ttgtggtgat ggtagccaga tggcaccggc agttgaagca accctgatga aaagctatgc
     3181 agatgttcat caggttagcg aagcagatgc acgtctgtgg ctgcaacagc tggaagaaaa
     3241 aggtcgttat gcaaaagatg tttgggcagg ttaataa
//