LOCUS       BBa_J119305              925 bp    DNA     linear       26-Jun-2024
DEFINITION  T5 promoter + Theo riboswitch + RFP
ACCESSION   BBa_J119305
VERSION     BBa_J119305.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa prefix
     promoter        23..75
                     /label=T5 promoter
     misc_feature    76..96
                     /label=lac operator
     misc_feature    97..102
                     /label=scar
     misc_feature    104..118
                     /label=constant sequence
     misc_feature    119..124
                     /label=KpnI site
     misc_feature    125..184
                     /label=Riboswitch
     RBS             168..172
                     /label=RBS
     misc_feature    182..184
                     /label=Start
     misc_feature    182..192
                     /label=GGA prefix ATGC sticky word
     misc_feature    193..213
                     /label=BBa suffix
     promoter        1..213
                     /label=BBa_J100065
     CDS             220..894
                     /label=mrfp1
     misc_feature    901..925
                     /label=Help:Barcodes
     CDS             220..925
                     /label=BBa_E1010
ORIGIN
        1 gaattcgcgg ccgcttctag agaaatcata aaaaatttat ttgctttgtg agcggataac
       61 aattataata gattcaattg tgagcggata acaattacta gagatacgac tcactatagg
      121 taccggtgat accagcatcg tcttgatgcc cttggcagca ccctgctaag gtaacaacaa
      181 gatgctgaga cctactagta gcggccgctg cagtactaga tggcttcctc cgaagacgtt
      241 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      301 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      361 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      421 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      481 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      541 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      601 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      661 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      721 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      781 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      841 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
      901 cgctgatagt gctagtgtag atcgc
//