BBa_J119306 1 BBa_J119306 RFP expression cassette for J-GGA 2013-07-01T11:00:00Z 2015-08-31T04:08:28Z Constructed by J-GGA from scaffold. The scaffold for Junction Golden Gate Assembly (J-GGA) is populated with a promoter related to P5 (J110031), a C dog RBS element related to BD2 (J119025), and a mRFP codon optimized for E. coli. J-GGA can be used to vary any of the elements. Inverted PCR from two junctions is followed by BsaI/Ligase assembly of elements carrying the appropriate BsaI sites or sticky ends. false false _613_120_ 0 606 61 Not in stock false None. false Sachii Polpitiya Arachchige annotation2330197 1 Junction C range2330197 1 151 168 annotation2330196 1 Junction B range2330196 1 54 71 annotation2330198 1 Junction A range2330198 1 1 18 annotation2330200 1 mRFP E coli range2330200 1 166 846 annotation2330202 1 C dog RBS range2330202 1 72 150 annotation2330201 1 Promoter range2330201 1 19 53 annotation2330199 1 Junction D range2330199 1 847 864 BBa_J119306_sequence 1 atagtctgttcatggtgcttgacaattaatatccggctcgtattttatgtggaggctgatgtagttaaggcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctacggaggtgttctaatggggaccatcgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacggagctatgtattcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z