LOCUS       BBa_J119313             1613 bp    DNA     linear       22-Nov-2024
DEFINITION  pClone Blue - Device for Testing New Promoters via Golden Gate 
            Assembly
ACCESSION   BBa_J119313
VERSION     BBa_J119313.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP reverse
     RBS             727..741
                     /label=RBS reverse
     misc_feature    755..758
                     /label=CGAC Sticky end
     misc_feature    844..847
                     /label=GCGG Sticky end
     misc_feature    760..765
                     /label=BsaI left
     misc_feature    837..842
                     /label=BsaI right
     promoter        766..836
                     /label=pLacIQ1 reverse
     RBS             861..947
                     /label=BD18 bicistron RBS
     CDS             945..1613
                     /label=AmilCP Blue
     misc_feature    1608..1613
                     /label=double stop
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactg agaccccggg cgctatcatg
      781 ccataccgcg aaaggttttg caccattcga tggtgtcaac gtaaatgcat gccgctggtc
      841 tctgcgggaa gacaactagg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      901 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatgagt gtgatcgcta
      961 aacaaatgac ctacaaagtg tatatgagcg gcaccgtgaa cggccattat tttgaagttg
     1021 aaggtgatgg taaaggcaaa ccgtatgaag gcgaacagac cgttaaactg accgttacca
     1081 aaggtggtcc gctgccgttt gcatgggata ttctgagtcc gcagtgtcag tatggtagca
     1141 ttccgtttac caaatatccg gaagatatcc cggattatgt gaaacagagc tttccggaag
     1201 gttatacctg ggaacgtatt atgaactttg aagatggtgc agtttgcacc gttagcaatg
     1261 atagcagcat tcagggtaat tgctttatct accacgtgaa atttagcggt ctgaattttc
     1321 cgcctaatgg tccggttatg cagaaaaaaa cccagggttg ggaaccgaat accgaacgtc
     1381 tgtttgcacg tgatggcatg ctgctgggta ataactttat ggcactgaaa ctggaaggtg
     1441 gtggtcatta tctgtgtgag tttaaaacca cctacaaagc caaaaaaccg gtgaaaatgc
     1501 ctggttatca ttatgtggat cgtaagctgg atgtgaccaa ccacaataaa gattacacca
     1561 gcgttgaaca gtgcgaaatt agcattgcac gcaaacctgt ggtcgcctaa taa
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