LOCUS BBa_J119318 4639 bp DNA linear 25-Nov-2024 DEFINITION eCDM8 expression + Theophylline riboswitch tetracycline resistance protein ACCESSION BBa_J119318 VERSION BBa_J119318.1 FEATURES Location/Qualifiers promoter 1..36 /label=J119031 RBS 37..124 /label=J119028 misc_feature 122..3274 /label=eCDM8 misc_feature 1..3274 /label=BBa_J119303 promoter 3283..3335 /label=T5 promoter misc_feature 3336..3356 /label=lac operator stem_loop 3405..3464 /label=riboswitch misc_feature 3442..3444 /label=Start codon CDS 3442..4632 /label=TetA tetracycline resistance gene misc_feature 4633..4635 /label=Stop codon misc_feature 3283..4639 /label=BBa_J119140 ORIGIN 1 ttgacaatta atcatccggc tcgtaattta tgtggagggc ccaagttcac ttaaaaagga 61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgcgatg gacggtttct 121 aatgaccatc aaagaaatgc cgcagccgaa aacctttggt gaactgaaaa atctgccgct 181 gctgaatacc gataaaccgg ttcatgcact gatgaaaatt gcagatgaac tgggcgagat 241 ctttaaattc gaagcaccgg gtagcgttac ccgttatctg agcagccagc gtctgattaa 301 agaagcatgc gacgaaagcc gctttgataa aaatctgatt cagtggctga aattcatccg 361 cgattttctg ggtgatggtc tgagcaccag ctggacccat gaaaaaaact ggaaaaaagc 421 ccataatatt ctgctgccga gctttagcca gcaggcaatg aaaggttatc atgcaatgat 481 ggttgatatt gccgttcagc tggttcagaa atgggaacgt ctgaatgcag atgaacatat 541 tgaagttccg gaagatatga cccgtctgac cctggatacc attggtctgt gtggttttaa 601 ctatcgcttc aacagctttt atcgtgatca gccgcatccg tttgttacca gcatggttcg 661 tgcactggat gaagcagtga ataaatggca gcgtgcaaat ccggatgatc cggcatatga 721 tgaaaataaa cgtcagttcc aagaggatat taaagtgatg aatgacctgg tggacaaaat 781 tatcgcagat cgtaaagcaa gcggtgaaca gagtgatgat ctgctgaccc atatgctgaa 841 tggtaaagat ccggaaaccg gtgaaccgct ggatgatgaa aacattcgtt atcagattat 901 taccttcctg attgccggtc atgaaaccac cagtggtctg ctgagctttg cactgtattt 961 tctggttaaa aatccgcacg ttctgcaaaa agcagccgaa gaagcagcac gcgttctggt 1021 tgatccggtt ccgagctata aacaggttaa acagctgaaa tatgtgggca tggttctgaa 1081 tgaagcactg cgtctgtggc cgacctttcc gtggtttagc ctgtatgcaa aagaagatac 1141 cgttctggga ggtgaatatc cgctggaaaa aggtgatgaa ctgatggttc tgattccgca 1201 gctgcatcgt gataaaacca tttggggtga tgatgtggaa gaatttcgtc cggaacgttt 1261 tgaaaatccg agcgcaattc cgcagcatgc ctttaaaccg tttggtaatg gtcagcgtgc 1321 atgtattggt cagcagtttg cactgcatga agccaccctg gttctgggta tgatgctgaa 1381 acattttgat tttgaggacc acaccaacta tgagctggat attaaaggca ccctgaccct 1441 gaaaccggaa ggttttgttg ttaaagccaa aagcaaaaaa atcccgctgg gtggtattcc 1501 gagcccgagc accgaacaga gcgcaaaaaa agttcgtaaa aaagccgaaa acgcccataa 1561 tacaccgctg ctggttctgt atggtagcaa tatgggtaca gccgaaggca ccgcacgtga 1621 tctggcagat attgcaatga gcaaaggttt tgcaccgcag gttgcaaccc tggatagcca 1681 tgcaggtaat ctgcctcgtg aaggtgcagt tctgattgtt accgcaagct ataatggtca 1741 tccgcctgat aatgcaaaac agtttgttga ttggctggat caggcaagcg cagatgaagt 1801 taaaggtgtt cgttatagcg tttttggttg cggtgataaa aactgggcaa ccacctatca 1861 gaaagttccg gcatttattg atgaaaccct ggcagcaaaa ggtgcagaaa atattgcaga 1921 tcgtggtgaa accgatgcca gtgatgattt tgaaggcacc tatgaagaat ggcgtgaaca 1981 tatgtggtca gatgttgcag cctattttaa cctggatatc gaaaacagcg aggacaataa 2041 aagcaccctg agcctgcaat ttgtggatag cgcagcagat atgccgctgg caaaaatgca 2101 tggtgcattt agcaccaatg ttgttgcaag caaagaactg caacagcctg gtagcgcacg 2161 tagcacccgt catctggaaa ttgaactgcc gaaagaagca agctatcaag agggtgatca 2221 tctgggtgtt attccgcgta attatgaagg tattgttaat cgtgttaccg cacgttttgg 2281 tctggatgca agccagcaga ttcgtctgga agcagaagaa gaaaaactgg cacatctgcc 2341 gctggccaaa accgttagcg ttgaagaact gctgcaatat gttgaactgc aagatccggt 2401 tacccgtacc cagctgcgtg caatggcagc aaaaaccgtt tgtccgcctc ataaagttga 2461 actggaagca ctgctggaga aacaggcata taaagaacag gttctggcaa aacgtctgac 2521 catgctggaa ctgctggaaa aatatccggc atgcgaaatg aaattcagcg aatttattgc 2581 actgctgccg agtattcgtc cgcgttatta tagcattagc agcagtccgc gtgttgatga 2641 aaaacaggcc agcattaccg ttagcgtggt tagcggtgaa gcatggtcag gttatggtga 2701 atataaaggt attgccagca attatctggc cgaactgcaa gaaggtgata ccattacctg 2761 ttttattagc acaccgcaga gcgaatttac cctgccgaaa gatcctgaaa caccgctgat 2821 tatggttggt ccgggtacag gtgttgcacc gtttcgtggt tttgttcagg cacgtaaaca 2881 actgaaagaa cagggtcaga gcctgggtga agcacatctg tattttggtt gtcgtagtcc 2941 gcatgaggat tatctgtatc aagaagaact ggaaaacgca cagagcgaag gtattattac 3001 cctgcatacc gcatttagcc gtatgccgaa tcagccgaaa acatatgttc agcatgttat 3061 ggaacaggat ggcaaaaaac tgattgaact gctggatcag ggtgcccatt tctatatttg 3121 tggtgatggt agccagatgg caccggcagt tgaagcaacc ctgatgaaaa gctatgcaga 3181 tgttcatcag gttagcgaag cagatgcacg tctgtggctg caacagctgg aagaaaaagg 3241 tcgttatgca aaagatgttt gggcaggtta ataatactag agaaatcata aaaaatttat 3301 ttgctttgtg agcggataac aattataata gattcaattg tgagcggata acaattacta 3361 gagatacgac tcactatagg taccggtgat accagcatcg tcttgatgcc cttggcagca 3421 ccctgctaag gtaacaacaa gatgcttaaa tctaacaatg cgctcatcgt catcctcggc 3481 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg 3541 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat 3601 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc 3661 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc 3721 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca 3781 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac 3841 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga 3901 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc 3961 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg 4021 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 4081 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 4141 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt 4201 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggccc cgccaccaaa 4261 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc 4321 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc 4381 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat 4441 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg 4501 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt 4561 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg 4621 gccacctcga cctaagcgt //