BBa_J119326 1 BBa_J119326 pTac mutant -10 TTTTT 2013-11-18T12:00:00Z 2015-08-31T04:08:28Z Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed. This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation converts the -10 region from TATAAT to TTTTTT. The mutation had a large negative effect on the function of the promoter (see Experience page). false false _613_435_ 0 606 61 Not in stock false Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed. false MWSU Genetics Fall 2013 Thursday 8:00 lab annotation2371412 1 -35 region range2371412 1 7 12 annotation2371413 1 mutant -10 region range2371413 1 29 34 BBa_J119326_sequence 1 gagctgttgacaattaatcatcggctcgttttttgtgtgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z