BBa_J119326
1
BBa_J119326
pTac mutant -10 TTTTT
2013-11-18T12:00:00Z
2015-08-31T04:08:28Z
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation converts the -10 region from TATAAT to TTTTTT. The mutation had a large negative effect on the function of the promoter (see Experience page).
false
false
_613_435_
0
606
61
Not in stock
false
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.
false
MWSU Genetics Fall 2013 Thursday 8:00 lab
annotation2371412
1
-35 region
range2371412
1
7
12
annotation2371413
1
mutant -10 region
range2371413
1
29
34
BBa_J119326_sequence
1
gagctgttgacaattaatcatcggctcgttttttgtgtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z