BBa_J119331 1 BBa_J119331 Ammeline Biosensor with Riboswitch AddA + RFP 2014-02-03T12:00:00Z 2015-08-31T04:08:28Z Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly. The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (AddA from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. false false _613_435_ 0 606 61 Not in stock false None. false Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige annotation2371786 1 P5 promoter range2371786 1 19 55 annotation2371785 1 AddA Ammeline riboswitch range2371785 1 73 188 annotation2371784 1 RFP range2371784 1 183 866 BBa_J119331_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataatcctaatgatatggtttgggagtttctaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttataaagagaagactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z