BBa_J119332
1
BBa_J119332
Ammeline Biosensor with Riboswitch M6 + RFP
2014-02-03T12:00:00Z
2015-08-31T04:08:28Z
Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly.
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.
false
false
_613_435_
0
606
61
Not in stock
false
None.
false
Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
annotation2371787
1
P5 promoter
range2371787
1
19
55
annotation2371788
1
RFP
range2371788
1
183
866
annotation2371789
1
M6 Ammeline riboswitch
range2371789
1
73
188
BBa_J119332_sequence
1
ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataatcctaatgatatggtttgggagcttccaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttataaagagaagactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactgcctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z