BBa_J119338 1 BBa_J119338 Ammeline Fitness Module with Riboswitch M6 + ThyA 2014-02-07T12:00:00Z 2015-08-31T04:08:28Z Mutagenesis of BBa_J119330 with inverse PCR followed by BsaI Golden Gate Assembly. The P5 promoter drives a riboswitch (M6 from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a ThyA (thymidylate synthase) fitness gene. false false _613_435_ 0 606 61 Not in stock false None. false Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige annotation2371809 1 ThyA range2371809 1 183 980 annotation2371808 1 M6 Ammleine riboswitch range2371808 1 73 88 annotation2371807 1 P5 promoter range2371807 1 19 55 BBa_J119338_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataatcctaatgatatggtttgggagcttccaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttataaagagaagactatgaagaaacagtatttagaactgatgcaaaaagtgctcgacgaaggcacacagaaaaacgaccgtaccggaaccggaacgctttccatttttggtcatcagatgcgttttaacctgcaagatggattcccgctggtgacaactaaacgttgccacctgcgttccatcatccatgaactgctgtggtttctacagggcgacactaacattgcttatctacacgaaaacaatgtcaccatctgggacgaatgggccgatgaaaacggcgacctcgggccagtgtatggtaaacagtggcgcgcctggccaacgccagatggtcgtcatattgaccagatcactacggtactgaaccagctgaaaaacgacccggattcgcgccgcattattgtttcagcgtggaacgtaggcgaactggataaaatggcgctggcaccgtgccatgcattcttccagttctatgtggcagacggcaaactctcttgccagctttatcagcgctcctgtgacgtcttcctcggcctgccgttcaacattgccagctacgcgttattggtgcatatgatggcgcagcagtgcgatctggaagtgggtgattttgtctggaccggtggcgacacgcatctgtacagcaaccatatggatcaaactcatctgcaattaagccgcgaaccgcgtccgctgccgaagttgattatcaaacgtaaacccgaatccatcttcgactaccgtttcgaagactttgagattgaaggctacgatccgcatccgggcattaaagcgccggtggctatctaagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z