BBa_J119341 1 BBa_J119341 Ammeline Biosensor with Riboswitch M6C 2014-02-14T12:00:00Z 2015-08-31T04:08:28Z Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly. The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. false false _613_435_ 0 606 61 Not in stock false None. false Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige annotation2371819 1 RFP range2371819 1 183 866 annotation2371817 1 P5 promoter range2371817 1 19 55 annotation2371818 1 M6C Ammeline riboswitch range2371818 1 73 188 BBa_J119341_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataatcctaatgatatggtttgggagcttccaccaagagccttaaactcttgactatgaagtctgtcgctttatccgaaattttataaagagaagactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z