LOCUS       BBa_J119342              870 bp    DNA     linear       23-Dec-2024
DEFINITION  Ammeline Biosensor with Riboswitch M6C prime (T167C)
ACCESSION   BBa_J119342
VERSION     BBa_J119342.1  
FEATURES             Location/Qualifiers
     promoter        19..55
                     /label=P5 promoter
     RBS             73..188
                     /label=M6C Ammeline riboswitch
     CDS             183..866
                     /label=RFP
     misc_feature    167..167
                     /label=T to C mutation
ORIGIN
        1 ccgtatacac agaggacctt gacaattaat catccggctc gtaatttatg tggatgggtc
       61 ttgaaccagg agtcaacgct tcatataatc ctaatgatat ggtttaggag cttccaccaa
      121 gagccttaaa ctcttgacta tgaagtctgt cgctttatcc gaaattctat aaagagaaga
      181 ctatgaaggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      241 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      301 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      361 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      421 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      481 tcgaagatgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      541 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
      601 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagatggt gctctgaaag
      661 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
      721 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
      781 tggacatcac ctcccacaac gaagattaca ccatcgttga acagtacgaa cgtgctgaag
      841 gtcgtcactc caccggtgct taataagcct
//