LOCUS BBa_J119345 5716 bp DNA linear 25-Nov-2024 DEFINITION RFP and eCDM8 expression + Theophylline riboswitch tetracycline resistance protein ACCESSION BBa_J119345 VERSION BBa_J119345.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..901 /label=mrfp1 misc_feature 908..932 /label=Help:Barcodes CDS 227..907 /label=BBa_E1010 stem_loop 952..995 /label=stem_loop misc_feature 941..1020 /label=BBa_B0010 terminator 941..1020 /label=BBa_B0010 stem_loop 1036..1055 /label=T7 TE misc_feature 1062..1062 /label=stop polyA_site 1056..1069 /label=polya misc_feature 1029..1069 /label=BBa_B0012 terminator 1029..1069 /label=BBa_B0012 misc_feature 1..1069 /label=BBa_J04450 promoter 1078..1113 /label=J119031 RBS 1114..1201 /label=J119028 misc_feature 1199..4351 /label=eCDM8 misc_feature 1078..4351 /label=BBa_J119303 promoter 4360..4412 /label=T5 promoter misc_feature 4413..4433 /label=lac operator stem_loop 4482..4541 /label=riboswitch misc_feature 4519..4521 /label=Start codon CDS 4519..5709 /label=TetA tetracycline resistance gene misc_feature 5710..5712 /label=Stop codon misc_feature 4360..5716 /label=BBa_J119140 misc_feature 1078..5716 /label=BBa_J119318 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga 241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttg 1081 acatcaggaa aatttttctg tataatgtgt ggagggccca agttcactta aaaaggagat 1141 caacaatgaa agcaattttc gtactgaaac atcttaatca tgctaaggag gttttctaat 1201 gaccatcaaa gaaatgccgc agccgaaaac ctttggtgaa ctgaaaaatc tgccgctgct 1261 gaataccgat aaaccggttc atgcactgat gaaaattgca gatgaactgg gcgagatctt 1321 taaattcgaa gcaccgggta gcgttacccg ttatctgagc agccagcgtc tgattaaaga 1381 agcatgcgac gaaagccgct ttgataaaaa tctgattcag tggctgaaat tcatccgcga 1441 ttttctgggt gatggtctga gcaccagctg gacccatgaa aaaaactgga aaaaagccca 1501 taatattctg ctgccgagct ttagccagca ggcaatgaaa ggttatcatg caatgatggt 1561 tgatattgcc gttcagctgg ttcagaaatg ggaacgtctg aatgcagatg aacatattga 1621 agttccggaa gatatgaccc gtctgaccct ggataccatt ggtctgtgtg gttttaacta 1681 tcgcttcaac agcttttatc gtgatcagcc gcatccgttt gttaccagca tggttcgtgc 1741 actggatgaa gcagtgaata aatggcagcg tgcaaatccg gatgatccgg catatgatga 1801 aaataaacgt cagttccaag aggatattaa agtgatgaat gacctggtgg acaaaattat 1861 cgcagatcgt aaagcaagcg gtgaacagag tgatgatctg ctgacccata tgctgaatgg 1921 taaagatccg gaaaccggtg aaccgctgga tgatgaaaac attcgttatc agattattac 1981 cttcctgatt gccggtcatg aaaccaccag tggtctgctg agctttgcac tgtattttct 2041 ggttaaaaat ccgcacgttc tgcaaaaagc agccgaagaa gcagcacgcg ttctggttga 2101 tccggttccg agctataaac aggttaaaca gctgaaatat gtgggcatgg ttctgaatga 2161 agcactgcgt ctgtggccga cctttccgtg gtttagcctg tatgcaaaag aagataccgt 2221 tctgggaggt gaatatccgc tggaaaaagg tgatgaactg atggttctga ttccgcagct 2281 gcatcgtgat aaaaccattt ggggtgatga tgtggaagaa tttcgtccgg aacgttttga 2341 aaatccgagc gcaattccgc agcatgcctt taaaccgttt ggtaatggtc agcgtgcatg 2401 tattggtcag cagtttgcac tgcatgaagc caccctggtt ctgggtatga tgctgaaaca 2461 ttttgatttt gaggaccaca ccaactatga gctggatatt aaaggcaccc tgaccctgaa 2521 accggaaggt tttgttgtta aagccaaaag caaaaaaatc ccgctgggtg gtattccgag 2581 cccgagcacc gaacagagcg caaaaaaagt tcgtaaaaaa gccgaaaacg cccataatac 2641 accgctgctg gttctgtatg gtagcaatat gggtacagcc gaaggcaccg cacgtgatct 2701 ggcagatatt gcaatgagca aaggttttgc accgcaggtt gcaaccctgg atagccatgc 2761 aggtaatctg cctcgtgaag gtgcagttct gattgttacc gcaagctata atggtcatcc 2821 gcctgataat gcaaaacagt ttgttgattg gctggatcag gcaagcgcag atgaagttaa 2881 aggtgttcgt tatagcgttt ttggttgcgg tgataaaaac tgggcaacca cctatcagaa 2941 agttccggca tttattgatg aaaccctggc agcaaaaggt gcagaaaata ttgcagatcg 3001 tggtgaaacc gatgccagtg atgattttga aggcacctat gaagaatggc gtgaacatat 3061 gtggtcagat gttgcagcct attttaacct ggatatcgaa aacagcgagg acaataaaag 3121 caccctgagc ctgcaatttg tggatagcgc agcagatatg ccgctggcaa aaatgcatgg 3181 tgcatttagc accaatgttg ttgcaagcaa agaactgcaa cagcctggta gcgcacgtag 3241 cacccgtcat ctggaaattg aactgccgaa agaagcaagc tatcaagagg gtgatcatct 3301 gggtgttatt ccgcgtaatt atgaaggtat tgttaatcgt gttaccgcac gttttggtct 3361 ggatgcaagc cagcagattc gtctggaagc agaagaagaa aaactggcac atctgccgct 3421 ggccaaaacc gttagcgttg aagaactgct gcaatatgtt gaactgcaag atccggttac 3481 ccgtacccag ctgcgtgcaa tggcagcaaa aaccgtttgt ccgcctcata aagttgaact 3541 ggaagcactg ctggagaaac aggcatataa agaacaggtt ctggcaaaac gtctgaccat 3601 gctggaactg ctggaaaaat atccggcatg cgaaatgaaa ttcagcgaat ttattgcact 3661 gctgccgagt attcgtccgc gttattatag cattagcagc agtccgcgtg ttgatgaaaa 3721 acaggccagc attaccgtta gcgtggttag cggtgaagca tggtcaggtt atggtgaata 3781 taaaggtatt gccagcaatt atctggccga actgcaagaa ggtgatacca ttacctgttt 3841 tattagcaca ccgcagagcg aatttaccct gccgaaagat cctgaaacac cgctgattat 3901 ggttggtccg ggtacaggtg ttgcaccgtt tcgtggtttt gttcaggcac gtaaacaact 3961 gaaagaacag ggtcagagcc tgggtgaagc acatctgtat tttggttgtc gtagtccgca 4021 tgaggattat ctgtatcaag aagaactgga aaacgcacag agcgaaggta ttattaccct 4081 gcataccgca tttagccgta tgccgaatca gccgaaaaca tatgttcagc atgttatgga 4141 acaggatggc aaaaaactga ttgaactgct ggatcagggt gcccatttct atatttgtgg 4201 tgatggtagc cagatggcac cggcagttga agcaaccctg atgaaaagct atgcagatgt 4261 tcatcaggtt agcgaagcag atgcacgtct gtggctgcaa cagctggaag aaaaaggtcg 4321 ttatgcaaaa gatgtttggg caggttaata atactagaga aatcataaaa aatttatttg 4381 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca attactagag 4441 atacgactca ctataggtac cggtgatacc agcatcgtct tgatgccctt ggcagcaccc 4501 tgctaaggta acaacaagat gcttaaatct aacaatgcgc tcatcgtcat cctcggcacc 4561 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg 4621 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg 4681 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc 4741 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca 4801 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 4861 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 4921 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 4981 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 5041 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc 5101 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 5161 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 5221 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 5281 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt 5341 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg 5401 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc 5461 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag 5521 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg 5581 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta 5641 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc 5701 acctcgacct aagcgt //