##gff-version 3
##sequence-region BBa_J119345 1 5716
BBa_J119345	.	engineered_region	1	1069	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_J119345	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_J119345	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J119345	.	terminator	1029	1069	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_J119345	.	stop_codon	1062	1062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J119345	.	stem_loop	1036	1055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J119345	.	polyA_site	1056	1069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J119345	.	engineered_region	1029	1069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J119345	.	CDS	227	907	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_J119345	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J119345	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J119345	.	terminator	941	1020	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_J119345	.	stem_loop	952	995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J119345	.	engineered_region	941	1020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J119345	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_J119345	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J119345	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J119345	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J119345	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J119345	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J119345	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J119345	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J119345	.	engineered_region	1078	5716	.	+	0	ID=BBa_J119318;Name=BBa_J119318
BBa_J119345	.	engineered_region	1078	4351	.	+	0	ID=BBa_J119303;Name=BBa_J119303;Parent=BBa_J119318
BBa_J119345	.	sequence_feature	1199	4351	.	+	0	ID=annotation2329362;Name=eCDM8;Parent=BBa_J119303
BBa_J119345	.	promoter	1078	1113	.	+	0	ID=annotation2329360;Name=J119031;Parent=BBa_J119303
BBa_J119345	.	ribosome_entry_site	1114	1201	.	+	0	ID=annotation2329361;Name=J119028;Parent=BBa_J119303
BBa_J119345	.	engineered_region	4360	5716	.	+	0	ID=BBa_J119140;Name=BBa_J119140;Parent=BBa_J119318
BBa_J119345	.	stem_loop	4482	4541	.	+	0	ID=annotation2214185;Name=riboswitch;Parent=BBa_J119140
BBa_J119345	.	start_codon	4519	4521	.	+	0	ID=annotation2214186;Name=Start codon;Parent=BBa_J119140
BBa_J119345	.	CDS	4519	5709	.	+	0	ID=annotation2214187;Name=TetA tetracycline resistance gene;Parent=BBa_J119140
BBa_J119345	.	stop_codon	5710	5712	.	+	0	ID=annotation2214188;Name=Stop codon;Parent=BBa_J119140
BBa_J119345	.	promoter	4360	4412	.	+	0	ID=annotation2214183;Name=T5 promoter;Parent=BBa_J119140
BBa_J119345	.	operator	4413	4433	.	+	0	ID=annotation2214184;Name=lac operator;Parent=BBa_J119140
>BBa_J119345
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacatcaggaaaatttttctgtataat
gtgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcta
aggaggttttctaatgaccatcaaagaaatgccgcagccgaaaacctttggtgaactgaaaaatctgccgctgctgaat
accgataaaccggttcatgcactgatgaaaattgcagatgaactgggcgagatctttaaattcgaagcaccgggtagcg
ttacccgttatctgagcagccagcgtctgattaaagaagcatgcgacgaaagccgctttgataaaaatctgattcagtg
gctgaaattcatccgcgattttctgggtgatggtctgagcaccagctggacccatgaaaaaaactggaaaaaagcccat
aatattctgctgccgagctttagccagcaggcaatgaaaggttatcatgcaatgatggttgatattgccgttcagctgg
ttcagaaatgggaacgtctgaatgcagatgaacatattgaagttccggaagatatgacccgtctgaccctggataccat
tggtctgtgtggttttaactatcgcttcaacagcttttatcgtgatcagccgcatccgtttgttaccagcatggttcgt
gcactggatgaagcagtgaataaatggcagcgtgcaaatccggatgatccggcatatgatgaaaataaacgtcagttcc
aagaggatattaaagtgatgaatgacctggtggacaaaattatcgcagatcgtaaagcaagcggtgaacagagtgatga
tctgctgacccatatgctgaatggtaaagatccggaaaccggtgaaccgctggatgatgaaaacattcgttatcagatt
attaccttcctgattgccggtcatgaaaccaccagtggtctgctgagctttgcactgtattttctggttaaaaatccgc
acgttctgcaaaaagcagccgaagaagcagcacgcgttctggttgatccggttccgagctataaacaggttaaacagct
gaaatatgtgggcatggttctgaatgaagcactgcgtctgtggccgacctttccgtggtttagcctgtatgcaaaagaa
gataccgttctgggaggtgaatatccgctggaaaaaggtgatgaactgatggttctgattccgcagctgcatcgtgata
aaaccatttggggtgatgatgtggaagaatttcgtccggaacgttttgaaaatccgagcgcaattccgcagcatgcctt
taaaccgtttggtaatggtcagcgtgcatgtattggtcagcagtttgcactgcatgaagccaccctggttctgggtatg
atgctgaaacattttgattttgaggaccacaccaactatgagctggatattaaaggcaccctgaccctgaaaccggaag
gttttgttgttaaagccaaaagcaaaaaaatcccgctgggtggtattccgagcccgagcaccgaacagagcgcaaaaaa
agttcgtaaaaaagccgaaaacgcccataatacaccgctgctggttctgtatggtagcaatatgggtacagccgaaggc
accgcacgtgatctggcagatattgcaatgagcaaaggttttgcaccgcaggttgcaaccctggatagccatgcaggta
atctgcctcgtgaaggtgcagttctgattgttaccgcaagctataatggtcatccgcctgataatgcaaaacagtttgt
tgattggctggatcaggcaagcgcagatgaagttaaaggtgttcgttatagcgtttttggttgcggtgataaaaactgg
gcaaccacctatcagaaagttccggcatttattgatgaaaccctggcagcaaaaggtgcagaaaatattgcagatcgtg
gtgaaaccgatgccagtgatgattttgaaggcacctatgaagaatggcgtgaacatatgtggtcagatgttgcagccta
ttttaacctggatatcgaaaacagcgaggacaataaaagcaccctgagcctgcaatttgtggatagcgcagcagatatg
ccgctggcaaaaatgcatggtgcatttagcaccaatgttgttgcaagcaaagaactgcaacagcctggtagcgcacgta
gcacccgtcatctggaaattgaactgccgaaagaagcaagctatcaagagggtgatcatctgggtgttattccgcgtaa
ttatgaaggtattgttaatcgtgttaccgcacgttttggtctggatgcaagccagcagattcgtctggaagcagaagaa
gaaaaactggcacatctgccgctggccaaaaccgttagcgttgaagaactgctgcaatatgttgaactgcaagatccgg
ttacccgtacccagctgcgtgcaatggcagcaaaaaccgtttgtccgcctcataaagttgaactggaagcactgctgga
gaaacaggcatataaagaacaggttctggcaaaacgtctgaccatgctggaactgctggaaaaatatccggcatgcgaa
atgaaattcagcgaatttattgcactgctgccgagtattcgtccgcgttattatagcattagcagcagtccgcgtgttg
atgaaaaacaggccagcattaccgttagcgtggttagcggtgaagcatggtcaggttatggtgaatataaaggtattgc
cagcaattatctggccgaactgcaagaaggtgataccattacctgttttattagcacaccgcagagcgaatttaccctg
ccgaaagatcctgaaacaccgctgattatggttggtccgggtacaggtgttgcaccgtttcgtggttttgttcaggcac
gtaaacaactgaaagaacagggtcagagcctgggtgaagcacatctgtattttggttgtcgtagtccgcatgaggatta
tctgtatcaagaagaactggaaaacgcacagagcgaaggtattattaccctgcataccgcatttagccgtatgccgaat
cagccgaaaacatatgttcagcatgttatggaacaggatggcaaaaaactgattgaactgctggatcagggtgcccatt
tctatatttgtggtgatggtagccagatggcaccggcagttgaagcaaccctgatgaaaagctatgcagatgttcatca
ggttagcgaagcagatgcacgtctgtggctgcaacagctggaagaaaaaggtcgttatgcaaaagatgtttgggcaggt
taataatactagagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcgg
ataacaattactagagatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgc
taaggtaacaacaagatgcttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgt
gctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgc
ccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctct
acgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgct
tcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctc
gcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttc
aaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaagcgt