LOCUS BBa_J119346 5716 bp DNA linear 26-Dec-2024 DEFINITION eCDM8 expression + Theophylline riboswitch tetracycline resistance protein + RFP expression ACCESSION BBa_J119346 VERSION BBa_J119346.1 FEATURES Location/Qualifiers promoter 1..36 /label=J119031 RBS 37..124 /label=J119028 misc_feature 122..3274 /label=eCDM8 misc_feature 1..3274 /label=BBa_J119303 promoter 3283..3335 /label=T5 promoter misc_feature 3336..3356 /label=lac operator stem_loop 3405..3464 /label=riboswitch misc_feature 3442..3444 /label=Start codon CDS 3442..4632 /label=TetA tetracycline resistance gene misc_feature 4633..4635 /label=Stop codon misc_feature 3283..4639 /label=BBa_J119140 misc_feature 1..4639 /label=BBa_J119318 CDS 4648..4735 /label=end of LacI coding region (inactive) misc_feature 4648..4847 /label=BBa_R0010 misc_feature 4736..4773 /label=CAP binding site promoter 4784..4789 /label=-35 promoter 4808..4813 /label=-10 misc_feature 4813..4847 /label=LacI binding site misc_feature 4820..4820 /label=start promoter 4648..4847 /label=BBa_R0010 misc_feature 4860..4863 /label=conserved RBS 4856..4867 /label=BBa_B0034 CDS 4874..5548 /label=mrfp1 misc_feature 5555..5579 /label=Help:Barcodes CDS 4874..5554 /label=BBa_E1010 stem_loop 5599..5642 /label=stem_loop misc_feature 5588..5667 /label=BBa_B0010 terminator 5588..5667 /label=BBa_B0010 stem_loop 5683..5702 /label=T7 TE misc_feature 5709..5709 /label=stop polyA_site 5703..5716 /label=polya misc_feature 5676..5716 /label=BBa_B0012 terminator 5676..5716 /label=BBa_B0012 misc_feature 4648..5716 /label=BBa_J04450 ORIGIN 1 ttgacaatta atcatccggc tcgtaattta tgtggagggc ccaagttcac ttaaaaagga 61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgcgatg gacggtttct 121 aatgaccatc aaagaaatgc cgcagccgaa aacctttggt gaactgaaaa atctgccgct 181 gctgaatacc gataaaccgg ttcatgcact gatgaaaatt gcagatgaac tgggcgagat 241 ctttaaattc gaagcaccgg gtagcgttac ccgttatctg agcagccagc gtctgattaa 301 agaagcatgc gacgaaagcc gctttgataa aaatctgatt cagtggctga aattcatccg 361 cgattttctg ggtgatggtc tgagcaccag ctggacccat gaaaaaaact ggaaaaaagc 421 ccataatatt ctgctgccga gctttagcca gcaggcaatg aaaggttatc atgcaatgat 481 ggttgatatt gccgttcagc tggttcagaa atgggaacgt ctgaatgcag atgaacatat 541 tgaagttccg gaagatatga cccgtctgac cctggatacc attggtctgt gtggttttaa 601 ctatcgcttc aacagctttt atcgtgatca gccgcatccg tttgttacca gcatggttcg 661 tgcactggat gaagcagtga ataaatggca gcgtgcaaat ccggatgatc cggcatatga 721 tgaaaataaa cgtcagttcc aagaggatat taaagtgatg aatgacctgg tggacaaaat 781 tatcgcagat cgtaaagcaa gcggtgaaca gagtgatgat ctgctgaccc atatgctgaa 841 tggtaaagat ccggaaaccg gtgaaccgct ggatgatgaa aacattcgtt atcagattat 901 taccttcctg attgccggtc atgaaaccac cagtggtctg ctgagctttg cactgtattt 961 tctggttaaa aatccgcacg ttctgcaaaa agcagccgaa gaagcagcac gcgttctggt 1021 tgatccggtt ccgagctata aacaggttaa acagctgaaa tatgtgggca tggttctgaa 1081 tgaagcactg cgtctgtggc cgacctttcc gtggtttagc ctgtatgcaa aagaagatac 1141 cgttctggga ggtgaatatc cgctggaaaa aggtgatgaa ctgatggttc tgattccgca 1201 gctgcatcgt gataaaacca tttggggtga tgatgtggaa gaatttcgtc cggaacgttt 1261 tgaaaatccg agcgcaattc cgcagcatgc ctttaaaccg tttggtaatg gtcagcgtgc 1321 atgtattggt cagcagtttg cactgcatga agccaccctg gttctgggta tgatgctgaa 1381 acattttgat tttgaggacc acaccaacta tgagctggat attaaaggca ccctgaccct 1441 gaaaccggaa ggttttgttg ttaaagccaa aagcaaaaaa atcccgctgg gtggtattcc 1501 gagcccgagc accgaacaga gcgcaaaaaa agttcgtaaa aaagccgaaa acgcccataa 1561 tacaccgctg ctggttctgt atggtagcaa tatgggtaca gccgaaggca ccgcacgtga 1621 tctggcagat attgcaatga gcaaaggttt tgcaccgcag gttgcaaccc tggatagcca 1681 tgcaggtaat ctgcctcgtg aaggtgcagt tctgattgtt accgcaagct ataatggtca 1741 tccgcctgat aatgcaaaac agtttgttga ttggctggat caggcaagcg cagatgaagt 1801 taaaggtgtt cgttatagcg tttttggttg cggtgataaa aactgggcaa ccacctatca 1861 gaaagttccg gcatttattg atgaaaccct ggcagcaaaa ggtgcagaaa atattgcaga 1921 tcgtggtgaa accgatgcca gtgatgattt tgaaggcacc tatgaagaat ggcgtgaaca 1981 tatgtggtca gatgttgcag cctattttaa cctggatatc gaaaacagcg aggacaataa 2041 aagcaccctg agcctgcaat ttgtggatag cgcagcagat atgccgctgg caaaaatgca 2101 tggtgcattt agcaccaatg ttgttgcaag caaagaactg caacagcctg gtagcgcacg 2161 tagcacccgt catctggaaa ttgaactgcc gaaagaagca agctatcaag agggtgatca 2221 tctgggtgtt attccgcgta attatgaagg tattgttaat cgtgttaccg cacgttttgg 2281 tctggatgca agccagcaga ttcgtctgga agcagaagaa gaaaaactgg cacatctgcc 2341 gctggccaaa accgttagcg ttgaagaact gctgcaatat gttgaactgc aagatccggt 2401 tacccgtacc cagctgcgtg caatggcagc aaaaaccgtt tgtccgcctc ataaagttga 2461 actggaagca ctgctggaga aacaggcata taaagaacag gttctggcaa aacgtctgac 2521 catgctggaa ctgctggaaa aatatccggc atgcgaaatg aaattcagcg aatttattgc 2581 actgctgccg agtattcgtc cgcgttatta tagcattagc agcagtccgc gtgttgatga 2641 aaaacaggcc agcattaccg ttagcgtggt tagcggtgaa gcatggtcag gttatggtga 2701 atataaaggt attgccagca attatctggc cgaactgcaa gaaggtgata ccattacctg 2761 ttttattagc acaccgcaga gcgaatttac cctgccgaaa gatcctgaaa caccgctgat 2821 tatggttggt ccgggtacag gtgttgcacc gtttcgtggt tttgttcagg cacgtaaaca 2881 actgaaagaa cagggtcaga gcctgggtga agcacatctg tattttggtt gtcgtagtcc 2941 gcatgaggat tatctgtatc aagaagaact ggaaaacgca cagagcgaag gtattattac 3001 cctgcatacc gcatttagcc gtatgccgaa tcagccgaaa acatatgttc agcatgttat 3061 ggaacaggat ggcaaaaaac tgattgaact gctggatcag ggtgcccatt tctatatttg 3121 tggtgatggt agccagatgg caccggcagt tgaagcaacc ctgatgaaaa gctatgcaga 3181 tgttcatcag gttagcgaag cagatgcacg tctgtggctg caacagctgg aagaaaaagg 3241 tcgttatgca aaagatgttt gggcaggtta ataatactag agaaatcata aaaaatttat 3301 ttgctttgtg agcggataac aattataata gattcaattg tgagcggata acaattacta 3361 gagatacgac tcactatagg taccggtgat accagcatcg tcttgatgcc cttggcagca 3421 ccctgctaag gtaacaacaa gatgcttaaa tctaacaatg cgctcatcgt catcctcggc 3481 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg 3541 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat 3601 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc 3661 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc 3721 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca 3781 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac 3841 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga 3901 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc 3961 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg 4021 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc 4081 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc 4141 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt 4201 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggccc cgccaccaaa 4261 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc 4321 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc 4381 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat 4441 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg 4501 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt 4561 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg 4621 gccacctcga cctaagcgtt actagagcaa tacgcaaacc gcctctcccc gcgcgttggc 4681 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 4741 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 4801 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga 4861 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt 4921 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg 4981 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt 5041 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc 5101 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt 5161 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg 5221 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat 5281 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc 5341 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga 5401 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga 5461 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg 5521 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct 5581 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5641 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5701 cctttctgcg tttata //