LOCUS BBa_J119347 5590 bp DNA linear 22-Nov-2024 DEFINITION eCDM8 expression + Theophylline riboswitch tetracycline resistance protein + GFP expression ACCESSION BBa_J119347 VERSION BBa_J119347.1 FEATURES Location/Qualifiers promoter 1..36 /label=J119030 RBS 37..124 /label=J119025 misc_feature 122..3277 /label=eCDM8 misc_feature 1..3277 /label=BBa_J119304 promoter 3286..3338 /label=T5 promoter misc_feature 3339..3359 /label=lac operator stem_loop 3408..3467 /label=riboswitch misc_feature 3445..3447 /label=Start codon CDS 3445..4635 /label=TetA tetracycline resistance gene misc_feature 4636..4638 /label=Stop codon misc_feature 3286..4642 /label=BBa_J119140 misc_feature 1..4642 /label=BBa_J119344 promoter 4662..4667 /label=-35 promoter 4687..4691 /label=-10 promoter 4651..4706 /label=BBa_K091112 RBS 4715..4727 /label=RBS-3\Weak RBS 4721..4724 /label=RBS misc_feature 4715..4727 /label=BBa_B0032 RBS 4715..4727 /label=BBa_B0032 CDS 4734..5453 /label=GFP protein CDS 4734..5453 /label=BBa_E0040 stem_loop 5473..5516 /label=stem_loop misc_feature 5462..5541 /label=BBa_B0010 terminator 5462..5541 /label=BBa_B0010 stem_loop 5557..5576 /label=T7 TE misc_feature 5583..5583 /label=stop polyA_site 5577..5590 /label=polya misc_feature 5550..5590 /label=BBa_B0012 terminator 5550..5590 /label=BBa_B0012 misc_feature 4651..5590 /label=BBa_K091131 ORIGIN 1 ttgacatcag gaaaattttt ctgtataatg tgtggagggc ccaagttcac ttaaaaagga 61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gaggttttct 121 aatgatgacc atcaaagaaa tgccgcagcc gaaaaccttt ggtgaactga aaaatctgcc 181 gctgctgaat accgataaac cggttcatgc actgatgaaa attgcagatg aactgggcga 241 gatctttaaa ttcgaagcac cgggtagcgt tacccgttat ctgagcagcc agcgtctgat 301 taaagaagca tgcgacgaaa gccgctttga taaaaatctg attcagtggc tgaaattcat 361 ccgcgatttt ctgggtgatg gtctgagcac cagctggacc catgaaaaaa actggaaaaa 421 agcccataat attctgctgc cgagctttag ccagcaggca atgaaaggtt atcatgcaat 481 gatggttgat attgccgttc agctggttca gaaatgggaa cgtctgaatg cagatgaaca 541 tattgaagtt ccggaagata tgacccgtct gaccctggat accattggtc tgtgtggttt 601 taactatcgc ttcaacagct tttatcgtga tcagccgcat ccgtttgtta ccagcatggt 661 tcgtgcactg gatgaagcag tgaataaatg gcagcgtgca aatccggatg atccggcata 721 tgatgaaaat aaacgtcagt tccaagagga tattaaagtg atgaatgacc tggtggacaa 781 aattatcgca gatcgtaaag caagcggtga acagagtgat gatctgctga cccatatgct 841 gaatggtaaa gatccggaaa ccggtgaacc gctggatgat gaaaacattc gttatcagat 901 tattaccttc ctgattgccg gtcatgaaac caccagtggt ctgctgagct ttgcactgta 961 ttttctggtt aaaaatccgc acgttctgca aaaagcagcc gaagaagcag cacgcgttct 1021 ggttgatccg gttccgagct ataaacaggt taaacagctg aaatatgtgg gcatggttct 1081 gaatgaagca ctgcgtctgt ggccgacctt tccgtggttt agcctgtatg caaaagaaga 1141 taccgttctg ggaggtgaat atccgctgga aaaaggtgat gaactgatgg ttctgattcc 1201 gcagctgcat cgtgataaaa ccatttgggg tgatgatgtg gaagaatttc gtccggaacg 1261 ttttgaaaat ccgagcgcaa ttccgcagca tgcctttaaa ccgtttggta atggtcagcg 1321 tgcatgtatt ggtcagcagt ttgcactgca tgaagccacc ctggttctgg gtatgatgct 1381 gaaacatttt gattttgagg accacaccaa ctatgagctg gatattaaag gcaccctgac 1441 cctgaaaccg gaaggttttg ttgttaaagc caaaagcaaa aaaatcccgc tgggtggtat 1501 tccgagcccg agcaccgaac agagcgcaaa aaaagttcgt aaaaaagccg aaaacgccca 1561 taatacaccg ctgctggttc tgtatggtag caatatgggt acagccgaag gcaccgcacg 1621 tgatctggca gatattgcaa tgagcaaagg ttttgcaccg caggttgcaa ccctggatag 1681 ccatgcaggt aatctgcctc gtgaaggtgc agttctgatt gttaccgcaa gctataatgg 1741 tcatccgcct gataatgcaa aacagtttgt tgattggctg gatcaggcaa gcgcagatga 1801 agttaaaggt gttcgttata gcgtttttgg ttgcggtgat aaaaactggg caaccaccta 1861 tcagaaagtt ccggcattta ttgatgaaac cctggcagca aaaggtgcag aaaatattgc 1921 agatcgtggt gaaaccgatg ccagtgatga ttttgaaggc acctatgaag aatggcgtga 1981 acatatgtgg tcagatgttg cagcctattt taacctggat atcgaaaaca gcgaggacaa 2041 taaaagcacc ctgagcctgc aatttgtgga tagcgcagca gatatgccgc tggcaaaaat 2101 gcatggtgca tttagcacca atgttgttgc aagcaaagaa ctgcaacagc ctggtagcgc 2161 acgtagcacc cgtcatctgg aaattgaact gccgaaagaa gcaagctatc aagagggtga 2221 tcatctgggt gttattccgc gtaattatga aggtattgtt aatcgtgtta ccgcacgttt 2281 tggtctggat gcaagccagc agattcgtct ggaagcagaa gaagaaaaac tggcacatct 2341 gccgctggcc aaaaccgtta gcgttgaaga actgctgcaa tatgttgaac tgcaagatcc 2401 ggttacccgt acccagctgc gtgcaatggc agcaaaaacc gtttgtccgc ctcataaagt 2461 tgaactggaa gcactgctgg agaaacaggc atataaagaa caggttctgg caaaacgtct 2521 gaccatgctg gaactgctgg aaaaatatcc ggcatgcgaa atgaaattca gcgaatttat 2581 tgcactgctg ccgagtattc gtccgcgtta ttatagcatt agcagcagtc cgcgtgttga 2641 tgaaaaacag gccagcatta ccgttagcgt ggttagcggt gaagcatggt caggttatgg 2701 tgaatataaa ggtattgcca gcaattatct ggccgaactg caagaaggtg ataccattac 2761 ctgttttatt agcacaccgc agagcgaatt taccctgccg aaagatcctg aaacaccgct 2821 gattatggtt ggtccgggta caggtgttgc accgtttcgt ggttttgttc aggcacgtaa 2881 acaactgaaa gaacagggtc agagcctggg tgaagcacat ctgtattttg gttgtcgtag 2941 tccgcatgag gattatctgt atcaagaaga actggaaaac gcacagagcg aaggtattat 3001 taccctgcat accgcattta gccgtatgcc gaatcagccg aaaacatatg ttcagcatgt 3061 tatggaacag gatggcaaaa aactgattga actgctggat cagggtgccc atttctatat 3121 ttgtggtgat ggtagccaga tggcaccggc agttgaagca accctgatga aaagctatgc 3181 agatgttcat caggttagcg aagcagatgc acgtctgtgg ctgcaacagc tggaagaaaa 3241 aggtcgttat gcaaaagatg tttgggcagg ttaataatac tagagaaatc ataaaaaatt 3301 tatttgcttt gtgagcggat aacaattata atagattcaa ttgtgagcgg ataacaatta 3361 ctagagatac gactcactat aggtaccggt gataccagca tcgtcttgat gcccttggca 3421 gcaccctgct aaggtaacaa caagatgctt aaatctaaca atgcgctcat cgtcatcctc 3481 ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc 3541 ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta 3601 tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc 3661 cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg 3721 accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc 3781 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc 3841 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg 3901 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc 3961 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg 4021 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 4081 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 4141 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 4201 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg ccccgccacc 4261 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac 4321 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct 4381 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac 4441 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga 4501 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg 4561 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc 4621 cgggccacct cgacctaagc gttactagag agcggcatgc atttacgttg acaccacctt 4681 tcgcggtatg gcatgatagc gcccggtact agagtcacac aggaaagtac tagatgcgta 4741 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 4801 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 4861 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 4921 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 4981 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 5041 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 5101 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 5161 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 5221 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 5281 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 5341 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 5401 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga 5461 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5521 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5581 tgcgtttata //