BBa_J119349 1 BBa_J119349 Ammeline Biosensor with Riboswitch D2 + RFP 2014-06-29T11:00:00Z 2015-08-31T04:08:28Z Riboswitch sequence TCAACACTTCATTTAATCCCGTGTATATGGTTTCGGAGCTTCCACCAAGAGCCTTAAACTCTTGACTATGAAGTCTGTCGCTTTATCCGAAATTTTATACACAGGATTGACTATGAAG The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (D2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The D2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The only differences between the M6C" riboswitch and D2 is the RBS and sequestration sequence location. false Kelly Cochran annotation2380008 1 RFP range2380008 1 185 868 annotation2380006 1 P5 promoter range2380006 1 19 55 annotation2380007 1 D2 Ammeline riboswitch range2380007 1 73 190 BBa_J119349_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacacttcatttaatcccgtgtatatggtttcggagcttccaccaagagccttaaactcttgactatgaagtctgtcgctttatccgaaattttatacacaggattgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z