BBa_J119351 1 BBa_J119351 Ammeline Biosensor with Riboswitch D5 + RFP 2014-06-29T11:00:00Z 2015-08-31T04:08:28Z This riboswitch is based on M6C". The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (D5) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The D5 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The D5 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and the sequestration sequence in the P2 stem of the aptamer. false Kelly Cochran annotation2380012 1 P5 promoter range2380012 1 19 55 annotation2380013 1 D5 Ammeline riboswitch range2380013 1 73 191 annotation2380014 1 RFP range2380014 1 186 869 BBa_J119351_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataatcctgtggatatcacgggcggagcttccaccaagagccttaaactcttgactatgaagtctgtcgctttatcagaaattttatacacaggattgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z