BBa_J119352 1 BBa_J119352 Ammeline Biosensor with Riboswitch N2 + RFP 2014-07-06T11:00:00Z 2015-08-31T04:08:28Z M6C" The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false Kelly Cochran annotation2380003 1 P5 promoter range2380003 1 19 55 annotation2380005 1 RFP range2380005 1 185 868 annotation2380004 1 N2 Ammeline riboswitch range2380004 1 73 190 BBa_J119352_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatttaatcctgtgtatatggtttcggagcttccaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttatacacaggattgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z