BBa_J119355 1 BBa_J119355 Ammeline Biosensor with Riboswitch D1 + RFP 2014-07-07T11:00:00Z 2015-08-31T04:08:28Z M6C" The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (D1) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The D1 riboswitch is based on the M6C" riboswitch with a RBS (AGGAGGT) designed by Davidson College. The sequestration sequence is available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The D1 riboswitch is based on the M6C" riboswitch with a RBS (AGGAGGT) designed by Davidson College. The sequestration sequence is available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false Kelly Cochran annotation2380022 1 D1 Ammeline riboswitch range2380022 1 73 190 annotation2380023 1 RFP range2380023 1 185 868 annotation2380021 1 P5 promoter range2380021 1 19 55 BBa_J119355_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacacttcatttaaacctccttatatggtttcggagcttccaccaagagccttaaactcttgactatgaagtctgtcgctttatccgaaattttataaggaggtttgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z