BBa_J119357 1 BBa_J119357 Ammeline Biosensor with Riboswitch D6 + RFP 2014-07-07T11:00:00Z 2015-08-31T04:08:28Z M6C" The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N4) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N4 riboswitch is based on the M6C" riboswitch with a RBS (AGGAGGT) designed by Davidson College and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The N4 riboswitch is based on the M6C" riboswitch with a RBS (AGGAGGT) designed by Davidson College and the sequestration sequence in the P2 stem of the aptamer (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false Kelly Cochran annotation2380028 1 D6 Ammeline riboswitch range2380028 1 73 190 annotation2380029 1 RFP range2380029 1 185 868 annotation2380027 1 P5 promoter range2380027 1 19 55 BBa_J119357_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatataaacctcctgatatggtttcggagcttccaccaagagccttaaactcttgactatgaagtctgtcgctttatccgaaattttataaggaggtttgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z