BBa_J119358 1 BBa_J119358 Ammeline Biosensor with Riboswitch N1 + RFP 2014-07-07T11:00:00Z 2015-08-31T04:08:28Z M6" The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N1) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N1 riboswitch is based on the M6" riboswitch with a RBS (AGGAGGT) designed by Davidson College. The sequestration sequence is available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false false _613_ 0 606 61 Not in stock false The N1 riboswitch is based on the M6" riboswitch with a RBS (AGGAGGT) designed by Davidson College. The sequestration sequence is available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). false Kelly Cochran annotation2380030 1 P5 promoter range2380030 1 19 55 annotation2380031 1 N1 Ammeline riboswitch range2380031 1 73 190 annotation2380032 1 RFP range2380032 1 185 868 BBa_J119358_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaacgcttcatttaaacctccttatatggtttcggagcttccaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttataaggaggtttgactatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z