LOCUS       BBa_J119361             1625 bp    DNA     linear       27-Dec-2024
DEFINITION  tClone Red: Device for Testing Transciptional Terminators and 
            Riboswitches via Golden Gate Assembly
ACCESSION   BBa_J119361
VERSION     BBa_J119361.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 promoter
     misc_feature    42..47
                     /label=BsaI left
     CDS             55..774
                     /label=GFP reverse
     RBS             781..795
                     /label=B0030 RBS reverse
     promoter        803..848
                     /label=P2 promoter reverse
     misc_feature    849..854
                     /label=BsaI right
     RBS             860..947
                     /label=BD18 C dog RBS
     CDS             945..1625
                     /label=RFP
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctctgcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      901 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggct tcctccgaag
      961 atgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     1021 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
     1081 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     1141 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     1201 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagatggt ggtgttgtta
     1261 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     1321 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1381 ccaccgaacg tatgtacccg gaagatggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1441 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     1501 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     1561 aagattacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     1621 aataa
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