LOCUS       BBa_J119363             1385 bp    DNA     linear       27-Dec-2024
DEFINITION  Ammeline Fitness Module with Riboswitch N2 + TetA (T85A, T89A, 
            G93C, T94C, G95T, T96G)
ACCESSION   BBa_J119363
VERSION     BBa_J119363.1  
FEATURES             Location/Qualifiers
     promoter        19..55
                     /label=P5 promoter
     RBS             73..190
                     /label=Ammeline Riboswitch
     CDS             185..1382
                     /label=TetA
     misc_feature    85..85
                     /label=T to A mutation
     misc_feature    89..89
                     /label=T to A mutation
     misc_feature    93..93
                     /label=G to C mutation
     misc_feature    94..94
                     /label=T to C mutation
     misc_feature    95..95
                     /label=G to T mutation
     misc_feature    96..96
                     /label=T to G mutation
ORIGIN
        1 ccgtatacac agaggacctt gacaattaat catccggctc gtaatttatg tggatgggtc
       61 ttgaaccagg agtcaacgct tcatataaac ctcctgatat ggtttcggag cttccaccaa
      121 gagccttaaa ctcttgatta tgaagtctgt cgctttatcc gaaattttat acacaggatt
      181 gactatgaag cttaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
      241 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
      301 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
      361 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
      421 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
      481 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      541 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      601 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      661 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
      721 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
      781 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
      841 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
      901 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
      961 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     1021 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     1081 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     1141 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     1201 aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc
     1261 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     1321 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta
     1381 agcct
//