LOCUS       BBa_J119374             2142 bp    DNA     linear       22-Dec-2024
DEFINITION  tClone TetA: Device for Testing Transciptional Terminators and 
            Riboswitches via Golden Gate Assembly
ACCESSION   BBa_J119374
VERSION     BBa_J119374.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 Promoter
     misc_feature    42..47
                     /label=BsaI Left
     CDS             55..774
                     /label=GFP
     RBS             781..792
                     /label=B0030 RBS rev
     promoter        803..848
                     /label=P2 Promoter rev
     misc_feature    849..854
                     /label=BsaI Right
     CDS             946..2142
                     /label=TetA
     RBS             861..946
                     /label=BD18 C Dog RBS
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctcctgcgg gggcccaagt tcacttaaaa aggagatcaa caatgaaagc
      901 aattttcgta ctgaaacatc ttaatcatgc gacggagcgt ttctaatggg acttaaatct
      961 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     1021 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     1081 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     1141 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     1201 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     1261 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     1321 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     1381 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     1441 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1501 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1561 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1621 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1681 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1741 gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     1801 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     1861 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     1921 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     1981 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     2041 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     2101 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aa
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