BBa_J119375 1 BBa_J119375 81 Mutated Ptac Promoters with Varying Strengths 2015-02-26T12:00:00Z 2015-08-31T04:08:28Z Synthetic oligonucleotides were cloned into pClone Red using BsaI Golden Gate Assembly. Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry. false false _613_ 0 606 61 Not in stock false None. false Anthony Eckdahl annotation2430524 1 -10 region range2430524 1 33 38 annotation2430526 1 NNNNNN range2430526 1 11 16 annotation2430525 1 -35 region range2430525 1 11 16 BBa_J119375_sequence 1 cgacgagctgttgacaattaatcatcggctcgtataatgtgtgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z