BBa_J119375
1
BBa_J119375
81 Mutated Ptac Promoters with Varying Strengths
2015-02-26T12:00:00Z
2015-08-31T04:08:28Z
Synthetic oligonucleotides were cloned into pClone Red using BsaI Golden Gate Assembly.
Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. The strength of each of 83 mutant promoters was measured by cloning them into pClone Red (J119137) and measuring RFP expression with fluorometry.
false
false
_613_
0
606
61
Not in stock
false
None.
false
Anthony Eckdahl
annotation2430524
1
-10 region
range2430524
1
33
38
annotation2430526
1
NNNNNN
range2430526
1
11
16
annotation2430525
1
-35 region
range2430525
1
11
16
BBa_J119375_sequence
1
cgacgagctgttgacaattaatcatcggctcgtataatgtgtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z