LOCUS BBa_J119386 2867 bp DNA linear 26-Nov-2024 DEFINITION tCloneTetRed; Fusion Protein Reporter ACCESSION BBa_J119386 VERSION BBa_J119386.1 FEATURES Location/Qualifiers promoter 1..36 /label=P5 promoter misc_feature 42..47 /label=BsaI site CDS 55..774 /label=GFP reverse promoter 803..848 /label=P2 promoter misc_feature 849..854 /label=BsaI site CDS 945..2138 /label=TetA protein half CDS 2139..2186 /label=Linker Sequence CDS 2187..2867 /label=RFP protein half RBS 781..792 /label=RBS B0034 RBS 860..945 /label=BD18 C Dog RBS ORIGIN 1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta 61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac 121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg 181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc 241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt 301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg 361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag 421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa 481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt 541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt 601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta 661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc 721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta 781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata 841 ctctttttgg tctctgcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca 901 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggga cttaaatcta 961 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg 1021 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc 1081 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg 1141 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca 1201 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca 1261 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 1321 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 1381 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 1441 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 1501 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 1561 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 1621 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggatcgc tttcgctgga 1681 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag 1741 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca 1801 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct 1861 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc 1921 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta 1981 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga 2041 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg 2101 cgttgcgtcg cggtgcatgg agccgagcca cctcgaccgg cggtggaagc ggcggcggct 2161 ccggtggtgg ttctggaggc ggttctatgg cttcctccga agatgttatc aaagagttca 2221 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 2281 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 2341 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 2401 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 2461 aatgggaacg tgttatgaac ttcgaagatg gtggtgttgt taccgttacc caggactcct 2521 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 2581 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 2641 cggaagatgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 2701 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 2761 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagattac accatcgttg 2821 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataa //