LOCUS       BBa_J119388             2781 bp    DNA     linear       29-Nov-2024
DEFINITION  rClone Tet Red: Device for GGA Cloning and Testing RBS elements 
            and Riboswitches
ACCESSION   BBa_J119388
VERSION     BBa_J119388.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5
     misc_feature    42..47
                     /label=BsaI Left
     CDS             55..774
                     /label=GFP Reverse
     RBS             781..792
                     /label=B0030 RBS reverse
     promoter        803..848
                     /label=P2 promoter reverse
     misc_feature    849..854
                     /label=BsaI right
     misc_feature    862..864
                     /label=Start codon
     CDS             862..2052
                     /label=TetA protein half
     CDS             2053..2100
                     /label=Linker Sequence
     CDS             2101..2781
                     /label=RFP protein half
     misc_feature    2779..2781
                     /label=Stop codon
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctctgcgga gatgcttaaa tctaacaatg cgctcatcgt catcctcggc
      901 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
      961 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat
     1021 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc
     1081 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc
     1141 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     1201 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     1261 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     1321 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     1381 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     1441 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     1501 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1561 tgggtcattt tcggcgagga tcgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     1621 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     1681 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     1741 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     1801 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     1861 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg
     1921 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     1981 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccga
     2041 gccacctcga ccggcggtgg aagcggcggc ggctccggtg gtggttctgg aggcggttct
     2101 atggcttcct ccgaagatgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
     2161 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
     2221 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
     2281 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
     2341 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
     2401 gatggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
     2461 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
     2521 atgggttggg aagcttccac cgaacgtatg tacccggaag atggtgctct gaaaggtgaa
     2581 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
     2641 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
     2701 atcacctccc acaacgaaga ttacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
     2761 cactccaccg gtgcttaata a
//