LOCUS       BBa_J119389             1530 bp    DNA     linear       23-Dec-2024
DEFINITION  rClone Blue: Device for GGA Cloning and Testing RBS elements and 
            Riboswitches
ACCESSION   BBa_J119389
VERSION     BBa_J119389.1  
FEATURES             Location/Qualifiers
     RBS             781..792
                     /label=B0034 RBS reverse
     CDS             55..774
                     /label=GFP reverse
     misc_feature    42..47
                     /label=BsaI left
     promoter        1..36
                     /label=P5 promoter
     misc_feature    849..854
                     /label=BsaI right
     CDS             862..1530
                     /label=Amil CP Blue
     promoter        803..848
                     /label=P2 promoter reverse
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctctgcgga gatgagcgtg attgcaaagc agatgaccta taaagtgtat
      901 atgagcggca ccgtgaacgg ccattatttt gaagttgaag gtgatggtaa aggcaaaccg
      961 tatgaaggcg aacagaccgt taaactgacc gttaccaaag gtggtccgct gccgtttgca
     1021 tgggatattc tgagtccgca gtgtcagtat ggtagcattc cgtttaccaa atatccggaa
     1081 gatatcccgg attatgtgaa acagagcttt ccggaaggtt atacctggga acgtattatg
     1141 aactttgaag atggtgcagt ttgcaccgtt agcaatgata gcagcattca gggtaattgc
     1201 tttatctacc acgtgaaatt tagcggtctg aattttccgc ctaatggtcc ggttatgcag
     1261 aaaaaaaccc agggttggga accgaatacc gaacgtctgt ttgcacgtga tggcatgctg
     1321 ctgggtaata actttatggc actgaaactg gaaggtggtg gtcattatct gtgtgagttt
     1381 aaaaccacct acaaagccaa aaaaccggtg aaaatgcctg gttatcatta tgtggatcgt
     1441 aaactggatg tgaccaacca caataaagat tacaccagcg ttgaacagtg cgaaattagc
     1501 attgcacgta aaccggttgt tgcctaataa
//