BBa_J119390 1 BBa_J119390 Collection of RBSs from N6 rClone Red Library 2016-04-28T11:00:00Z 2016-04-29T08:46:49Z Synthetic oligos cloned into rClone Red (BBa_J119384). Oligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384)to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths. false false _613_435_ 606 606 61 false The oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly. false Anthony J. Eckdahl annotation2479249 1 misc range2479249 1 1 8 BBa_J119390_sequence 1 cgcgaggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z