BBa_J119393
1
BBa_J119393
Collection of Bicistronic RBSs from N8 rClone Red Library
2016-04-28T11:00:00Z
2016-04-29T10:35:53Z
Synthetic oligos cloned into rClone Red (BBa_J119384).
Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red ([http://parts.igem.org/Part:BBa_J119384 BBa_J119384]). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.
false
false
_613_120_
606
606
61
false
The oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly.
false
Anthony J. Eckdahl
BBa_J119393_sequence
1
gggggggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z