LOCUS       BBa_J119394             3657 bp    DNA     linear       09-May-2024
DEFINITION  tClone TetA+SacB: Device for Testing Transciptional Terminators 
            and Riboswitches via GGA
ACCESSION   BBa_J119394
VERSION     BBa_J119394.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5
     misc_feature    42..47
                     /label=BsaI Left
     RBS             781..792
                     /label=B0030 RBS rev
     CDS             55..774
                     /label=GFP
     promoter        803..848
                     /label=P2 Promoter rev
     misc_feature    849..854
                     /label=BsaI Right
     RBS             861..946
                     /label=BD18 C Dog RBS
     CDS             946..2142
                     /label=TetA
     CDS             2180..3657
                     /label=SacB
     RBS             2148..2182
                     /label=BD18
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttgg tctcctgcgg gggcccaagt tcacttaaaa aggagatcaa caatgaaagc
      901 aattttcgta ctgaaacatc ttaatcatgc gacggagcgt ttctaatggg acttaaatct
      961 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     1021 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     1081 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     1141 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     1201 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     1261 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     1321 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     1381 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     1441 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1501 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1561 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1621 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1681 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1741 gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     1801 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     1861 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     1921 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     1981 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     2041 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     2101 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aactagaggg cccaagttca
     2161 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgcgac
     2221 ggagcgtttc taatgaacat caaaaaattc gcaaaacagg caaccgttct gacctttacc
     2281 accgcactgc tggcaggcgg tgcaacccag gcatttgcaa aagaaaccaa tcagaaaccg
     2341 tataaagaaa cgtatggcat cagccatatt acccgtcatg atatgctgca aattccggaa
     2401 cagcagaaaa acgaaaaata tcaggtgccc gaatttgata gcagcacgat taaaaacatt
     2461 agcagcgcaa aaggtctgga tgtttgggat agctggccgt tacagaatgc agatggcacc
     2521 gttgcaaatt atcatggcta tcacattgtt tttgccttag ccggtgatcc gaaaaatgcc
     2581 gatgatacca gcatctatat gttttatcag aaagtgggcg aaaccagcat tgacagctgg
     2641 aaaaacgcag gtcgtgtgtt taaagatagc gataaatttg atgccaacga tagcatcctg
     2701 aaagatcaga cccaagaatg gtcaggtagc gcaaccttta caagtgatgg taaaatccgt
     2761 ctgttctata ccgatttcag cggtaaacat tatggtaaac agaccctgac cacagcacag
     2821 gttaatgtta gcgcaagcga tagcagtctg aatattaacg gtgtggaaga ttacaaaagc
     2881 atctttgatg gtgatggcaa aacctaccag aatgtgcagc agtttattga tgaaggcaat
     2941 tatagcagcg gtgataatca taccctgcgt gatccgcatt atgttgaaga taaaggccat
     3001 aaatatctgg tgtttgaagc aaataccggc accgaagatg gttatcaggg tgaagaaagc
     3061 ctgtttaaca aagcctatta tggcaaatcg accagctttt ttcgtcaaga aagtcagaaa
     3121 ctgttacaga gcgacaaaaa acgtaccgca gaactggcaa atggtgcact gggcatgatt
     3181 gaactgaatg atgattacac cctgaaaaaa gtgatgaaac cgctgattgc aagcaatacc
     3241 gtgaccgatg aaattgaacg tgccaatgtg ttcaaaatga acggcaagtg gtatttattc
     3301 accgatagcc gtggtagcaa aatgaccatt gatggtatta cgagcaacga catttatatg
     3361 ctgggttatg tgagcaatag tctgaccggt ccttataaac ctctgaataa aaccggtctg
     3421 gtgctgaaaa tggatctgga tccgaatgat gtgacattta cctatagcca ttttgcagtt
     3481 ccgcaggcca aaggtaataa tgttgttatt accagctaca tgaccaaccg tggtttttat
     3541 gcagataaac agagcacctt tgcaccgagc tttttactga acattaaagg caagaaaacc
     3601 agcgttgtga aagacagcat tctggaacag ggacagctga ccgtgaataa gtaataa
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