BBa_J119394 1 BBa_J119394 tClone TetA+SacB: Device for Testing Transciptional Terminators and Riboswitches via GGA 2016-06-22T11:00:00Z 2016-06-28T02:04:31Z Existing par BBa_J119374 and PCR product of SacB coding sequence. tClone TetA+SacB can be used to clone and test new transcriptional terminators and riboswitches that function by antitermination without gel purification or other preparation of DNA. A new terminator or riboswitch can be derived from synthetic oligos, PCR, or a plasmid clone and cloned into tClone TetA with Golden Gate Assembly (GGA) using BsaI and ligase. For proper assembly, see BBa_J119367. Whether or not transcription proceeds to the TetA gene is determined by the strength of the terminator or whether termination or antitermination occurs at the riboswitch. false false _613_435_ 606 606 61 false No BsaI sites in SacB coding sequence. false Athena J. Davis annotation2480797 1 SacB range2480797 1 2180 3657 annotation2480622 1 BsaI Right range2480622 1 849 854 annotation2480617 1 P5 range2480617 1 1 36 annotation2480806 1 BD18 range2480806 1 2148 2182 annotation2480618 1 BsaI Left range2480618 1 42 47 annotation2480619 1 B0030 RBS rev range2480619 1 781 792 annotation2480624 1 BD18 C Dog RBS range2480624 1 861 946 annotation2480620 1 GFP range2480620 1 55 774 annotation2480625 1 TetA range2480625 1 946 2142 annotation2480621 1 P2 Promoter rev range2480621 1 803 848 BBa_J119394_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgactgagaccactagagttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaattactagatccacacattataggtacaaaaagatgcgaagtcaatactctttttggtctcctgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgggacttaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaactagagggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgaacatcaaaaaattcgcaaaacaggcaaccgttctgacctttaccaccgcactgctggcaggcggtgcaacccaggcatttgcaaaagaaaccaatcagaaaccgtataaagaaacgtatggcatcagccatattacccgtcatgatatgctgcaaattccggaacagcagaaaaacgaaaaatatcaggtgcccgaatttgatagcagcacgattaaaaacattagcagcgcaaaaggtctggatgtttgggatagctggccgttacagaatgcagatggcaccgttgcaaattatcatggctatcacattgtttttgccttagccggtgatccgaaaaatgccgatgataccagcatctatatgttttatcagaaagtgggcgaaaccagcattgacagctggaaaaacgcaggtcgtgtgtttaaagatagcgataaatttgatgccaacgatagcatcctgaaagatcagacccaagaatggtcaggtagcgcaacctttacaagtgatggtaaaatccgtctgttctataccgatttcagcggtaaacattatggtaaacagaccctgaccacagcacaggttaatgttagcgcaagcgatagcagtctgaatattaacggtgtggaagattacaaaagcatctttgatggtgatggcaaaacctaccagaatgtgcagcagtttattgatgaaggcaattatagcagcggtgataatcataccctgcgtgatccgcattatgttgaagataaaggccataaatatctggtgtttgaagcaaataccggcaccgaagatggttatcagggtgaagaaagcctgtttaacaaagcctattatggcaaatcgaccagcttttttcgtcaagaaagtcagaaactgttacagagcgacaaaaaacgtaccgcagaactggcaaatggtgcactgggcatgattgaactgaatgatgattacaccctgaaaaaagtgatgaaaccgctgattgcaagcaataccgtgaccgatgaaattgaacgtgccaatgtgttcaaaatgaacggcaagtggtatttattcaccgatagccgtggtagcaaaatgaccattgatggtattacgagcaacgacatttatatgctgggttatgtgagcaatagtctgaccggtccttataaacctctgaataaaaccggtctggtgctgaaaatggatctggatccgaatgatgtgacatttacctatagccattttgcagttccgcaggccaaaggtaataatgttgttattaccagctacatgaccaaccgtggtttttatgcagataaacagagcacctttgcaccgagctttttactgaacattaaaggcaagaaaaccagcgttgtgaaagacagcattctggaacagggacagctgaccgtgaataagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z