BBa_J119396 1 BBa_J119396 SacB + PDB riboswitch 2016-06-23T11:00:00Z 2016-06-24T09:35:06Z Existing part BBa_J119042 and annealed oligos containing the PDB riboswitch sequence. The PDB riboswitch consists of a theophylline aptamer sequence from the Protein Databank. This aptamer sequence was inserted into the Wachsmuth et al. RS3 riboswitch sequence using Golden Gate Assembly. The SacB plasmid backbone was obtained from part J119042, and Golden Gate Assembly was used to ligate the riboswitch into the SacB plasmid. false false _613_435_ 606 606 61 false None false Athena J. Davis annotation2480763 1 PDB riboswitch range2480763 1 55 126 annotation2480762 1 SacB range2480762 1 212 1636 annotation2480759 1 pTet range2480759 1 1 52 annotation2480761 1 BD18 bicistronic junction range2480761 1 127 214 BBa_J119396_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgaacatcaaaaaattcgcaaaacaggcaaccgttctgacctttaccaccgcactgctggcaggcggtgcaacccaggcatttgcaaaagaaaccaatcagaaaccgtataaagaaacgtatggcatcagccatattacccgtcatgatatgctgcaaattccggaacagcagaaaaacgaaaaatatcaggtgcccgaatttgatagcagcacgattaaaaacattagcagcgcaaaaggtctggatgtttgggatagctggccgttacagaatgcagatggcaccgttgcaaattatcatggctatcacattgtttttgccttagccggtgatccgaaaaatgccgatgataccagcatctatatgttttatcagaaagtgggcgaaaccagcattgacagctggaaaaacgcaggtcgtgtgtttaaagatagcgataaatttgatgccaacgatagcatcctgaaagatcagacccaagaatggtcaggtagcgcaacctttacaagtgatggtaaaatccgtctgttctataccgatttcagcggtaaacattatggtaaacagaccctgaccacagcacaggttaatgttagcgcaagcgatagcagtctgaatattaacggtgtggaagattacaaaagcatctttgatggtgatggcaaaacctaccagaatgtgcagcagtttattgatgaaggcaattatagcagcggtgataatcataccctgcgtgatccgcattatgttgaagataaaggccataaatatctggtgtttgaagcaaataccggcaccgaagatggttatcagggtgaagaaagcctgtttaacaaagcctattatggcaaatcgaccagcttttttcgtcaagaaagtcagaaactgttacagagcgacaaaaaacgtaccgcagaactggcaaatggtgcactgggcatgattgaactgaatgatgattacaccctgaaaaaagtgatgaaaccgctgattgcaagcaataccgtgaccgatgaaattgaacgtgccaatgtgttcaaaatgaacggcaagtggtatttattcaccgatagccgtggtagcaaaatgaccattgatggtattacgagcaacgacatttatatgctgggttatgtgagcaatagtctgaccggtccttataaacctctgaataaaaccggtctggtgctgaaaatggatctggatccgaatgatgtgacatttacctatagccattttgcagttccgcaggccaaaggtaataatgttgttattaccagctacatgaccaaccgtggtttttatgcagataaacagagcacctttgcaccgagctttttactgaacattaaaggcaagaaaaccagcgttgtgaaagacagcattctggaacagggacagctgaccgtgaataagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z