LOCUS BBa_J119397 5432 bp DNA linear 23-Dec-2024 DEFINITION pJC173b gIII neg (Carlson et al. 2014) ACCESSION BBa_J119397 VERSION BBa_J119397.1 FEATURES Location/Qualifiers CDS 1..951 /label=RepA misc_feature 955..1700 /label=SC101 Origin of Replication primer_bind 1785..1804 /label=T7 promoter 1785..1804 /label=T7 Promoter RBS 1826..1840 /label=SD8 RBS CDS 1841..2162 /label=geneIII neg CDS 2158..4281 /label=LuxAB CDS 4361..5221 /label=AmpR promoter 5222..5416 /label=bla promoter ORIGIN 1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag 61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc 121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt 181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg 241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa 301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa 361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt 421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt 481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc 541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg 601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt 661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat 721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat 781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat 841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc 901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa 961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact 1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact 1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta 1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga 1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc 1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac 1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga 1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg 1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag 1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct 1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta 1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca 1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga 1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctactaatac gactcactat 1801 agggagaaag acctgcaggt gcagtaagga ggaaaaaaaa atgaaaaaat tattattcgc 1861 aattccttta gttgttcctt tctattctca ctccgctgaa actgtttccc aaatggctca 1921 agtcggtgac ggtgataatt cacctttaat gaataatttc cgtcaatatt taccttccct 1981 ccctcaatcg gttgaatgtc gcccttttgt ctttggcgct ggtaaacctt acgagttcag 2041 tatcgactgc gataagatca acctgttccg cggtgtcttt gcgtttcttt tatatgttgc 2101 cacctttatg tatgtatttt ctacgtttgc taacatactg cgtaataagg agtcttaatg 2161 aaatttggaa actttttgct tacataccaa cctccccaat tttcccaaac agaggtaatg 2221 aaacgtttgg ttaaattagg tcgcatctct gaggagtgtg gttttgatac cgtatggtta 2281 ctggagcatc atttcacgga gtttggtttg cttggtaacc cttatgtcgc tgctgcatat 2341 ttacttggcg cgactaaaaa attgaatgta ggaactgccg ctattgttct tcccacagcc 2401 catccagtac gccaacttga agatgtgaat ttattggatc aaatgtcaaa aggacgattt 2461 cggtttggta tttgccgagg gctttacaac aaggactttc gcgtattcgg cacagatatg 2521 aataacagtc gcgccttagc ggaatgctgg tacgggctga taaagaatgg catgacagag 2581 ggatatatgg aagctgataa tgaacatatc aagttccata aggtaaaagt aaaccccgcg 2641 gcgtatagca gaggtggcgc accggtttat gtggtggctg aatcagcttc gacgactgag 2701 tgggctgctc aatttggcct accgatgata ttaagttgga ttataaatac taacgaaaag 2761 aaagcacaac ttgagcttta taatgaagtg gctcaagaat atgggcacga tattcataat 2821 atcgaccatt gcttatcata tataacatct gtagatcatg actcaattaa agcgaaagag 2881 atttgccgga aatttctggg gcattggtat gattcttatg tgaatgctac gactattttt 2941 gatgattcag accaaacaag aggttatgat ttcaataaag ggcagtggcg tgactttgta 3001 ttaaaaggac ataaagatac taatcgccgt attgattaca gttacgaaat caatcccgtg 3061 ggaacgccgc aggaatgtat tgacataatt caaaaagaca ttgatgctac aggaatatca 3121 aatatttgtt gtggatttga agctaatgga acagtagacg aaattattgc ttccatgaag 3181 ctcttccagt ctgatgtcat gccatttctt aaagaaaaac aacgttcgct attatattat 3241 ggcggtggcg gtagcggcgg tggcggtagc ggcggtggcg gtagcggcgg tggcggtagc 3301 aaatttggat tgttcttcct taacttcatc aattcaacaa ctgttcaaga acagagtata 3361 gttcgcatgc aggaaataac ggagtatgtt gataagttga attttgaaca gattttagtg 3421 tatgaaaatc atttttcaga taatggtgtt gtcggcgctc ctctgactgt ttctggtttt 3481 ctgctcggtt taacagagaa aattaaaatt ggttcattaa atcacatcat tacaactcat 3541 catcctgtcc gcatagcgga ggaagcttgc ttattggatc agttaagtga agggagattt 3601 attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc atttttttaa tcgcccggtt 3661 gaatatcaac agcaactatt tgaagagtgt tatgaaatca ttaacgatgc tttaacaaca 3721 ggctattgta atccagataa cgatttttat agcttcccta aaatatctgt aaatccccat 3781 gcttatacgc caggcggacc tcggaaatat gtaacagcaa ccagtcatca tattgttgag 3841 tgggcggcca aaaaaggtat tcctctcatc tttaagtggg atgattctaa tgatgttaga 3901 tatgaatatg ctgaaagata taaagccgtt gcggataaat atgacgttga cctatcagag 3961 atagaccatc agttaatgat attagttaac tataacgaag atagtaataa agctaaacaa 4021 gagacgcgtg catttattag tgattatgtt cttgaaatgc accctaatga aaatttcgaa 4081 aataaacttg aagaaataat tgcagaaaac gctgtcggaa attatacgga gtgtataact 4141 gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg tattgctgtc ctttgaacca 4201 atgaatgatt tgatgagcca aaaaaatgta atcaatattg ttgatgataa tattaagaag 4261 taccacacgg aatataccta aacttaatta acggcactcc tcagcaaata taatgaccct 4321 cttgataacc caagagggca ttttttaatg cccatggcgt ttaccaatgc ttaatcagtg 4381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 4981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5341 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 5401 gtatcacgag gcccttaggc caaataggcc gt //