BBa_J119400 1 BBa_J119400 tCloneTet+PDB riboswitch with P5 and BD10 2016-07-17T11:00:00Z 2016-07-18T09:22:25Z J100255 is the original tClone Tet+PDB Riboswitch and it is from Campbell M Lab. J119026, located in Eckdahl Lab, includes the BD10 C Dog RBS. The BD10 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD10 was inserted via Decoupled Golden Gate Assembly. false false _613_ 606 606 61 false BD10 was inserted via Decoupled Golden Gate Assembly. iPCR amplified everything on the J100255 plasmid, besides BD18. false Avery Umphreys annotation2481008 1 p5 range2481008 1 102 137 annotation2481010 1 TetA range2481010 1 307 726 annotation2481009 1 BD10 range2481009 1 222 305 BBa_J119400_sequence 1 gccggaaaaatgagcgattctcgatgagctgacccgatcgccgccgccggagggttgcgtttgagacgggcgacagatgaattcgcggccgcttctagagttgacaattaatcatccggctcgtaatttatgtggacgacggcgataccagccgaaaggcccttggcagcgtctttacatactcggtaaacgacgctgccaaggttttttttgcggcgacgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggaggatcgtttctaatgggacttaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagggcttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z